Antibodies and Proteins Tech Tips
ANTIBODIES
Chemists' Technique Reveals Whether Antibodies Neutralize SARS-CoV-2
GlycoSeLect provided the MIT team with Recombinant Prokaryotic Lectins. These RPLS assisted Prof. Laura Kiessling (MIT Novartis Professor of Chemistry and founding Editor of ACS Chemical Biology) and her team with their research and findings. We are grateful for the opportunity and proud to be...
A Guide to Understanding Lectins vs Antibodies
Both lectins and antibodies are powerful binding proteins used across many applications. Since their discovery in the late 19th century, these molecules have been widely studied, classified, and utilized in the advancement of research, health, and the sciences...
Considerations When Selecting a Primary Antibody
Antibodies are blood proteins produced in the immune system in response to a specific antigen. This specific antigenicity is what makes antibodies such a powerful tool in immunohistochemistry. The earliest reference to antibodies goes back to the 1890s...
The Right Tool for the Job: Selecting Appropriate Protein Detection and Visualisation Methods
Since the method was first described by Albert Coons in 1941, immunostaining has been a foundational tool in understanding the mechanisms underlying health and disease. In this article,
Pam James, PhD, Vice President, Product, Vector Laboratories...
PROTEOMICS
Unlocking the Histone Code: A Spotlight on Three Essential Histone PTMs
The "histone code" hypothesis postits that epigenetic features, specifically histone post-transitional modifications (PTMs), act as a unique molecular language to regulate chromatin structure and gene expression 1-3. But how does this work?...
Many components of the biotechnology and medical toolbox have their origins in pathogenic microorganisms. These have been adapted to generate useful tools and therapies for research and medicine. Now Caf1 proteins, also known as Cytotoxic Assocation Factor 1...
Affinity purification (also called affinity chromatography) is recognized as the most powerful method of purification chromatography or enriching a protein of interest from a complex mixture such as a crude cell lysate, cell culture supernatant, or other samples...
SPRI Bead or Column DNA Purification Which Method is Best for CUT&RUN?
Purification of target-enriched DNA is central to CUT&RUN (and CUT&Tag).chromatin mapping workflows. When considering a DNA cleanup strategy, multiple methods are available: phenol-chloroform extraction, column-based separation, and SPRI bead technology. With so many options, how can you besure you've selected the right method?