{"title":"10040-Mutant Nucs","description":"","products":[{"product_id":"nucleosome-recombinant-human-acidic-patch-mutant-h2ae61a-biotinylated-ship-product-on-dry-ice","title":"**Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE61A Biotinylated","description":"\u003cul\u003e\n\u003cli\u003eMononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A*, H2B, H3 and H4; accession numbers: H2A-P04908*; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA [1] containing a 5’ biotin-TEG group. \u003c\/li\u003e\n\u003cli\u003e*Histone H2A contains a glutamate-to-alanine (E-to-A) substitution at position 61 (H2AE61A). H2AE61A is among key residues forming a negatively charged region on the nucleosome surface named the “acidic patch”. The acidic patch is a conserved interaction hub for neighboring nucleosomes and nucleosome binding proteins, often via salt bridges with arginine anchors, and is functionally critical in chromatin condensation and chromatin remodeling [2-4]. H2AE61 resides in the alpha2 helix and forms a key salt bridge with H4K16. H2AE61 mediates chromatin binding with factors such as LANA, RCC1, IL-33, SIR3 and HMGN2 [2]. H2AE61A disrupts chromatin remodeling by the ISWI remodeler SNF2h [4].\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"EpiCypher","offers":[{"title":"Default Title","offer_id":53612310561059,"sku":"EP160029","price":810.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/2021.05.05_acidic_patch_nuc_RGB_white_border_LS_icon_thumbnail_board_2__67572.1621809858.png?v=1752978106"},{"product_id":"nucleosome-recombinant-human-acidic-patch-mutant-h2ae92k-biotinylated-ship-product-on-dry-ice","title":"**Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE92K Biotinylated","description":"\u003cul\u003e\n\u003cli\u003eMononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A*, H2B, H3 and H4; accession numbers: H2A-P04908*; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA [1] containing a 5’ biotin-TEG group. \u003c\/li\u003e\n\u003cli\u003e*Histone H2A contains a glutamate-to-lysine (E-to-K) substitution at position 92 (H2AE92K). H2AE92 is among key residues forming a negatively charged region on the nucleosome surface named the “acidic patch”. The acidic patch is a conserved interaction hub for neighboring nucleosomes and nucleosome binding proteins, often via salt bridges with arginine anchors, and is functionally critical in chromatin condensation and chromatin remodeling [2-4]. H2AE92 resides in the H2A C-terminal extension and is associated with nucleosome binding factors such as histone H4 N-terminal tail, LANA, RCC1, IL-33, SIR3, HMGN2, and remodeling ATPases [2-4]. H2AE92K disrupts binding with SMARCB1 [5].\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"EpiCypher","offers":[{"title":"Default Title","offer_id":53612310593827,"sku":"EP160030","price":810.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/2021.05.05_acidic_patch_nuc_RGB_white_border_LS_icon_thumbnail_board_2__67572.1621809858_abcf30cf-da47-4fc3-9ad7-b8842c3c6ce9.png?v=1752978110"},{"product_id":"nucleosome-recombinant-human-acidic-patch-mutant-h2be105a-e113a-biotinylated-ship-product-on-dry-ice","title":"**Nucleosome, Recombinant Human, Acidic Patch Mutant H2BE105A,E113A Biotinylated","description":"\u003cul\u003e\n\u003cli\u003eMononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B*, H3 and H4; accession numbers: H2A-P04908; H2B-O60814*; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA [1] containing a 5’ biotin-TEG group. \u003c\/li\u003e\n\u003cli\u003e*Histone H2B contains a glutamate-to-alanine (E-to-A) substitution at positions 105 and 113 (H2BE105A,E113A). H2BE105 and H2BE113 are among key residues forming a negatively charged region on the nucleosome surface named the “acidic patch”. The acidic patch is a conserved interaction hub for neighboring nucleosomes and nucleosome binding proteins, often via salt bridges with arginine anchors, and is functionally critical in chromatin condensation and chromatin remodeling [2-4]. H2B E105 and E113 both reside in H2B alphaC extension and are associated with nucleosome binding factors such as histone H4 N-terminal tail, LANA, RCC1, HMGN2 and SMARCB1 [2,5].\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"EpiCypher","offers":[{"title":"Default Title","offer_id":53612310626595,"sku":"EP160031","price":810.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/2021.05.05_acidic_patch_nuc_RGB_white_border_LS_icon_thumbnail_board_2__67572.1621809858_08769fff-62ac-4a3a-ad15-378f0a1a79a8.png?v=1752978113"},{"product_id":"acidic-patch-mutant-h2ae61a-recombinant-nucleosome-with-linker-dna-biotinylated-50-ug-ship-product-on-dry-ice","title":"**Acidic Patch Mutant H2AE61A Recombinant Nucleosome with Linker DNA, Biotinylated, 50 µg","description":"\u003cp\u003eThe acidic patch is a negatively charged region of the nucleosome surface that serves as a conserved interaction hub for neighboring nucleosomes and chromatin binding proteins, often via salt bridges with arginine anchors [1]. The acidic patch plays a critical role in chromatin condensation and chromatin remodeling. Recombinant mononucleosomes containing acidic patch mutations can be used to study the biological functions of the acidic patch.\u003c\/p\u003e\u003cp\u003eH2AE61A Recombinant Nucleosome with Linker DNA consists of 199 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 5’ biotin-TEG DNA consists of a core 147 bp 601 nucleosome assembly sequence [4] flanked by 26 bp linker sequences as underlined below. Histone H2A contains a glutamate-to-alanine (E-to-A) substitution within the acidic patch at position 61 (H2AE61A). H2AE61 resides in the alpha2 helix, forming a key salt bridge with H4K16, and mediates chromatin binding with factors such as LANA, RCC1, IL-33, Sir3, and HMGN2. H2AE61A disrupts chromatin remodeling by SMARCA5\/SNF2h, an ATPase subunit of the ISWI family of chromatin remodeling complexes.\u003c\/p\u003e","brand":"EpiCypher","offers":[{"title":"Default Title","offer_id":53612315246883,"sku":"EP162029","price":810.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/2021.05.05_acidic_patch_nuc_RGB_white_border_LS_icon_thumbnail_board_2__41959.1717092961.png?v=1752978273"},{"product_id":"acidic-patch-mutant-h2ae92k-recombinant-nucleosome-with-linker-dna-biotinylated-ship-product-on-dry-ice","title":"**Acidic Patch Mutant H2AE92K Recombinant Nucleosome with Linker DNA, Biotinylated","description":"\u003cp class=\"richp\"\u003eThe acidic patch is a negatively charged region of the nucleosome surface that serves as a conserved interaction hub for neighboring nucleosomes and chromatin binding proteins, often via salt bridges with arginine anchors [1]. The acidic patch plays a critical role in chromatin condensation and chromatin remodeling. Recombinant mononucleosomes containing acidic patch mutations can be used to study the biological functions of the acidic patch.\u003c\/p\u003e\u003cp class=\"richp\"\u003eH2AE92K Recombinant Nucleosome with Linker DNA consists of 199 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 5’ biotin-TEG DNA consists of a core 147 bp 601 nucleosome assembly sequence flanked by 26 bp linker sequences as underlined below. Histone H2A contains a glutamate-to-lysine (E-to-K) substitution at position 92 (H2AE92K). H2AE92 resides in the H2A C-terminal extension and mediates chromatin binding with factors such as H4-N-terminal tail, LANA, RCC1, IL-33, Sir3, HMGN2, and SMARCA5\/SNF2h, an ATPase subunit of the ISWI family of chromatin remodeling complexes. H2AE92K disrupts binding with SMARCB1\/BAF47, a subunit of the SWI\/SNF (BAF) family of chromatin remodeling complexes. These complexes serve a critical role in cell division, cell and tissue differentiation, and development, and SWI\/SNF complex malfunction has been linked to over 20% of human cancers.\u003c\/p\u003e","brand":"EpiCypher","offers":[{"title":"Default Title","offer_id":53612315279651,"sku":"EP162030","price":0.01,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/2021.05.05_acidic_patch_nuc_RGB_white_border_LS_icon_thumbnail_board_2__41959__47696.1717694541.png?v=1752978277"},{"product_id":"acidic-patch-mutant-h2be105a-e113a-recombinant-nucleosome-with-linker-dna-biotinylated-50-ug-ship-product-on-dry-ice","title":"**Acidic Patch Mutant H2BE105A,E113A Recombinant Nucleosome with Linker DNA, Biotinylated, 50 µg","description":"\u003cp\u003eThe acidic patch is a negatively charged region of the nucleosome surface that serves as a conserved interaction hub for neighboring nucleosomes and nucleosome binding proteins, often via salt bridges with arginine anchors. The acidic patch plays a critical role in chromatin condensation and chromatin remodeling. Recombinant mononucleosomes containing acidic patch mutations can be used to study the biological functions of the acidic patch.\u003c\/p\u003e\u003cp\u003eH2BE105A,E113A Recombinant Nucleosome with Linker DNA consists of 199 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 5’ biotin-TEG DNA consists of a core 147 bp 601 nucleosome assembly sequence flanked by 26 bp linker sequences as underlined below. Histone H2B contains a glutamate-to-alanine (E-to-A) substitution at positions 105 and 113 (H2BE105A, E113A). H2BE105 and H2BE113 both reside in H2B alphaC helix extension and are associated with nucleosome binding factors such as H4-N-terminal tail, LANA, RCC1, and HMGN2. H2BE105A,E113A disrupts binding with SMARCB1\/BAF47, a subunit of the SWI\/SNF (BAF) family of chromatin remodeling complexes. These complexes serve a critical role in cell division, cell and tissue differentiation, and development, and SWI\/SNF complex malfunction has been linked to over 20% of human cancers.\u003c\/p\u003e","brand":"EpiCypher","offers":[{"title":"Default Title","offer_id":53612315312419,"sku":"EP162031","price":810.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/2021.05.05_acidic_patch_nuc_RGB_white_border_LS_icon_thumbnail_board_2__84943.1716971267.png?v=1752978280"}],"url":"https:\/\/chromspec.com\/collections\/10040-mutant-nucs.oembed","provider":"Chromatographic Specialties Inc.","version":"1.0","type":"link"}