{"title":"10043-Mutant Nucs","description":"","products":[{"product_id":"nucleosome-recombinant-human-acidic-patch-mutant-h2ae61a-ship-product-on-dry-ice","title":"**Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE61A","description":"\u003cul\u003e\n\u003cli\u003eMononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A*, H2B, H3 and H4; accession numbers: H2A-P04908*; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA [1]. \u003c\/li\u003e\n\u003cli\u003e*Histone H2A contains a glutamate-to-alanine (E-to-A) substitution at position 61 (H2AE61A). H2AE61A is among key residues forming a negatively charged region on the nucleosome surface named the “acidic patch”. The acidic patch is a conserved interaction hub for neighboring nucleosomes and nucleosome binding proteins, often via salt bridges with arginine anchors, and is functionally critical in chromatin condensation and chromatin remodeling [2-4]. H2AE61 resides in the alpha2 helix and forms a key salt bridge with H4K16. H2AE61 mediates chromatin binding with factors such as LANA, RCC1, IL-33, SIR3 and HMGN2 [2]. H2AE61A disrupts chromatin remodeling by the ISWI remodeler SNF2h [4].\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"EpiCypher","offers":[{"title":"Default Title","offer_id":53612314558755,"sku":"EP161029","price":810.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/2021.05.05_acidic_patch_nuc_RGB_white_border_LS_icon_thumbnail_board_2__67572.1621809858_2955eff8-db1b-4903-934c-3751c0057609.png?v=1752978317"},{"product_id":"nucleosome-recombinant-human-acidic-patch-mutant-h2ae92k-ship-product-on-dry-ice","title":"**Nucleosome, Recombinant Human, Acidic Patch Mutant H2AE92K","description":"\u003cul\u003e\n\u003cli\u003eMononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A*, H2B, H3 and H4; accession numbers: H2A-P04908*; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA [1]. \u003c\/li\u003e\n\u003cli\u003e*Histone H2A contains a glutamate-to-lysine (E-to-K) substitution at position 92 (H2AE92K). H2AE92 is among key residues forming a negatively charged region on the nucleosome surface named the “acidic patch”. The acidic patch is a conserved interaction hub for neighboring nucleosomes and nucleosome binding proteins, often via salt bridges with arginine anchors, and is functionally critical in chromatin condensation and chromatin remodeling [2-4]. H2AE92 resides in the H2A C-terminal extension and is associated with nucleosome binding factors such as histone H4 N-terminal tail, LANA, RCC1, IL-33, SIR3, HMGN2, and remodeling ATPases [2-4]. H2AE92K disrupts binding with SMARCB1 [5].\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"EpiCypher","offers":[{"title":"Default Title","offer_id":53612314591523,"sku":"EP161030","price":810.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/2021.05.05_acidic_patch_nuc_RGB_white_border_LS_icon_thumbnail_board_2__67572.1621809858_00de32e2-585d-4f1b-adc5-f71be5eae17f.png?v=1752978321"},{"product_id":"nucleosome-recombinant-human-acidic-patch-mutant-h2be105a-e113a-ship-product-on-dry-ice","title":"**Nucleosome, Recombinant Human, Acidic Patch Mutant H2BE105A,E113A","description":"\u003cul\u003e\n\u003cli\u003eMononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B*, H3 and H4; accession numbers: H2A-P04908; H2B-O60814*; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA [1]. \u003c\/li\u003e\n\u003cli\u003e*Histone H2B contains a glutamate-to-alanine (E-to-A) substitution at positions 105 and 113 (H2BE105A,E113A). H2BE105 and H2BE113 are among key residues forming a negatively charged region on the nucleosome surface named the “acidic patch”. The acidic patch is a conserved interaction hub for neighboring nucleosomes and nucleosome binding proteins, often via salt bridges with arginine anchors, and is functionally critical in chromatin condensation and chromatin remodeling [2-4]. H2B E105 and E113 both reside in the H2B alphaC extension and are associated with nucleosome binding factors such as histone H4 N-terminal tail, LANA, RCC1, HMGN2 and SMARCB1 [2,5].\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"EpiCypher","offers":[{"title":"Default Title","offer_id":53612314624291,"sku":"EP161031","price":810.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/2021.05.05_acidic_patch_nuc_RGB_white_border_LS_icon_thumbnail_board_2__67572.1621809858_9efc0d0c-d0cc-4ac2-848f-14cdf6d75b87.png?v=1752978324"}],"url":"https:\/\/chromspec.com\/collections\/10043-mutant-nucs.oembed","provider":"Chromatographic Specialties Inc.","version":"1.0","type":"link"}