{"title":"14903-HNP1-3","description":"","products":[{"product_id":"hnp1-3-human-elisa-kit-1-x-96-det","title":"HNP1-3, Human, ELISA kit, 1 x 96 det.","description":"\u003cul\u003e\n\u003cli\u003eHuman neutrophil defensins (alpha-defensins, HNP1-3) belong to the family of cationic trisulfide-containing microbicidal peptides. Besides microbicidal, the peptides exert chemotactic, immunomodulating and cytotoxic activity and participate in host defense and inflammation. Azurophilic granules of neutrophils contain Human Neutrophil Peptide (HNP)-1-4 which are highly homologous. The three principal human defensins, HNP 1-3, are unique to neutrophils and account for about 99 percent of the total defensin content of these cells. Measured amount of defensins is 3-5 mg per million human neutrophils. Activation of neutrophils leads to rapid release of defensins. Thus, only one cell type, neutrophils, may be the source of HNP1-3 measured in plasma and other body fluids during infection and inflammation. In normal plasma low levels of HNP are present ranging from undetectable level to 50-100 ng\/ml, while in septic conditions the levels of HNP might be elevated to 10 mg\/ml and even more. Activation of neutrophils in blood as occurs during clotting, as well as long storage of anticoagulated blood leads to a release of HNP, thus careful plasma sampling is important for possible detection of HNP. Defensins are relatively resistant to proteolysis, low pH and boiling, but have a tendency to bind to a variety of materials, including plastic and proteins. The HNP 1-3 ELISA shows cross reactivity with Rhesus monkey HNP 1-3.\u003c\/li\u003e\n\u003cli\u003eThe human HNP1-3 ELISA has been developed for the quantitative measurement of natural and recombinant human HNP1-3 in cell culture medium, plasma and other biological fluids. In plasma samples human HNP1-3 can be measured accurately if plasma samples are diluted at least 2000 times for human and at least 4x for Rhesus monkey.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Hycult Biotech","offers":[{"title":"Default Title","offer_id":53614112768291,"sku":"HYBHK31701","price":1379.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/Hycult_20800x800_20100dpi_446e648f-6f2a-47dd-8935-03dae8e0e40d.png?v=1753030262"},{"product_id":"hnp1-3-human-elisa-kit-2-x-96-det","title":"HNP1-3, Human, ELISA kit, 2 x 96 det.","description":"\u003cul\u003e\n\u003cli\u003eHuman neutrophil defensins (alpha-defensins, HNP1-3) belong to the family of cationic trisulfide-containing microbicidal peptides. Besides microbicidal, the peptides exert chemotactic, immunomodulating and cytotoxic activity and participate in host defense and inflammation. Azurophilic granules of neutrophils contain Human Neutrophil Peptide (HNP)-1-4 which are highly homologous. The three principal human defensins, HNP 1-3, are unique to neutrophils and account for about 99 percent of the total defensin content of these cells. Measured amount of defensins is 3-5 mg per million human neutrophils. Activation of neutrophils leads to rapid release of defensins. Thus, only one cell type, neutrophils, may be the source of HNP1-3 measured in plasma and other body fluids during infection and inflammation. In normal plasma low levels of HNP are present ranging from undetectable level to 50-100 ng\/ml, while in septic conditions the levels of HNP might be elevated to 10 mg\/ml and even more. Activation of neutrophils in blood as occurs during clotting, as well as long storage of anticoagulated blood leads to a release of HNP, thus careful plasma sampling is important for possible detection of HNP. Defensins are relatively resistant to proteolysis, low pH and boiling, but have a tendency to bind to a variety of materials, including plastic and proteins. The HNP 1-3 ELISA shows cross reactivity with Rhesus monkey HNP 1-3.\u003c\/li\u003e\n\u003cli\u003eThe human HNP1-3 ELISA has been developed for the quantitative measurement of natural and recombinant human HNP1-3 in cell culture medium, plasma and other biological fluids. In plasma samples human HNP1-3 can be measured accurately if plasma samples are diluted at least 2000 times for human and at least 4x for Rhesus monkey.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Hycult Biotech","offers":[{"title":"Default Title","offer_id":53614112801059,"sku":"HYBHK31702","price":2271.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/Hycult_20800x800_20100dpi_bfaa7e85-f879-4452-b42d-bff540589bb7.png?v=1753030265"}],"url":"https:\/\/chromspec.com\/collections\/14903-hnp1-3.oembed","provider":"Chromatographic Specialties Inc.","version":"1.0","type":"link"}