{"title":"15186-MD-2","description":"","products":[{"product_id":"md-2-human-mab-18h10","title":"MD-2, Human, mAb 18H10","description":"\u003cul\u003e\n\u003cli\u003eThe monoclonal antibody 18H10 reacts with MD-2, an accessory molecule of the Toll-like receptor 4 (TLR4, CD284). TLRs belong to a family of proteins that specifically recognizes and senses microbial products. They are highly conserved throughout evolution and act as innate immune recognition receptors against many pathogens. TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4. MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomers and multimers (sMD-2). Circulating sMD-2 is mainly present as a doublet of ~20 and 25 kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells. The monoclonal antibody 18H10 reacts with MD-2. However, it does not react with sMD-2. In addition, the monoclonal antibody 18H10 is able to inhibit bacterial binding to MD-2.\u003c\/li\u003e\n\u003cli\u003eApplications: Flow Cytometry , Functional Studies\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Hycult Biotech","offers":[{"title":"Default Title","offer_id":53614151565603,"sku":"HYBHM2245100UG","price":645.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/Hycult_20800x800_20100dpi_412d700f-cf59-451a-b1af-3b40a3ec7472.png?v=1753031878"},{"product_id":"md-2-human-mab-18h10-20-ug","title":"MD-2, Human, mAb 18H10, 20 µg","description":" ","brand":"Hycult Biotech","offers":[{"title":"Default Title","offer_id":53614151598371,"sku":"HYBHM224520UG","price":265.9,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/Hycult_20800x800_20100dpi_840028b2-491b-45c5-b5b4-dcafba6e9277.png?v=1753031881"}],"url":"https:\/\/chromspec.com\/collections\/15186-md-2.oembed","provider":"Chromatographic Specialties Inc.","version":"1.0","type":"link"}