{"title":"15575-Avidin and Biotinylated Reagents","description":"","products":[{"product_id":"avidin-d-10-mg","title":"Avidin D, 10 mg","description":"\u003cp\u003eAvidin D, 10 mg, is isolated from egg white by a specific affinity system which results in a homogeneous, ultrapure avidin with very low non-specific binding characteristics. Avidin D is recommended for use in solid-phase assays and many histochemical applications when the technique of sequential addition of reagents (\"sandwich\" technique) is employed.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53617459986723,"sku":"VECTA2000","price":283.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/a-2000-10_avidin_d-jpg.webp?v=1753117816"},{"product_id":"fluorescein-avidin-d-fitc","title":"Fluorescein Avidin D (FITC)","description":"\u003cp\u003eFluorescein Avidin D, FITC, 5 mg, having an excitation at 495 nm and an emission at about 515 nm, is made from Vector Laboratories' highly purified Avidin D by a special labeling technique. This product has an optimal fluorochrome to protein ratio and is the reagent we recommend for routine immunofluorescence applications. Compared to conventional primary and secondary fluorescent labeling techniques, Fluorescein Avidin D can provide greater sensitivity and lower background staining. (For even higher sensitivity and specific applications, see Fluorescein Avidin DCS, Cell Sorting Grade, (Cat. No. A-2011-1).\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53617460052259,"sku":"VECTA2001","price":373.7,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/a-2001-5_fluorescein_avidind_fitc.webp?v=1753117820"},{"product_id":"horseradish-peroxidase-avidin-d","title":"Horseradish Peroxidase Avidin D","description":"\u003cp\u003eHorseradish Peroxidase Avidin D (Av-HRP), Concentrate, for IHC, 5 mg, is produced by Vector Laboratories' own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled Avidin D, antigens can be localized in histological sections, cytospin preparations, or smears.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53617460183331,"sku":"VECTA2004","price":376.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/a-2004-5_hrp_avidin_d_ihc-jpg.webp?v=1753117824"},{"product_id":"fluorescein-avidin-dcs","title":"Fluorescein Avidin DCS","description":"\u003cp\u003eFluorescein Avidin DCS, Cell Sorting Grade, 1 mg, is a cell sorter grade of Fluorescein Avidin D. Through the development of a new method of fluorescein labeling, Vector Laboratories is able to incorporate more fluorochrome into a protein without altering the properties of the protein. Not only does the Fluorescein Avidin DCS have an optimal F\/P ratio, the efficiency of fluorescence is also increased. In addition to the improved conjugation technique, Vector employs a purification step to reduce non-specific binding, which can hamper resolution in cell sorting and other applications. Although this product has been designed for cell sorting, it can also be used in fluorescent microscopy when higher sensitivity and lower background staining are necessary. This derivative is preferred when using the amplification procedure with Biotinylated Anti-Avidin D. Fluorescein Avidin DCS has an excitation at 495 nm and an emission at about 515 nm.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53617460314403,"sku":"VECTA2011","price":350.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/a-2011-1_fluorescein_avidin_dcs-jpg.webp?v=1753117831"},{"product_id":"horseradish-peroxidase-avidin-d-elisa-grade","title":"Horseradish Peroxidase Avidin D, ELISA Grade","description":"\u003cp\u003eHorseradish Peroxidase Avidin D (Av-HRP), Concentrate, for ELISAs and Blots, 5 mg, is optimized for use in solid-phase assays such as ELISAs and blotting applications. Vector Laboratories enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53617460478243,"sku":"VECTA2014","price":354.7,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/a-2014-5_hrp_avidin_d_elisas_blots-1.webp?v=1753117835"},{"product_id":"alkaline-phosphatase-avidin-d-100-units","title":"Alkaline Phosphatase Avidin D, 100 Units","description":"\u003cp\u003eAlkaline Phosphatase Avidin D, 100U, is a powerful tool to detect or purify biotinylated proteins, nucleic acids, and other macromolecules. Vector Laboratories enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates. These enzyme conjugates are suitable for use in solid-phase assays, tissue\/cell staining systems, and blotting applications. One unit is 1 mole p-nitrophenylphosphate hydrolyzed per minute at 37 degrees C, pH 9.8.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53617460642083,"sku":"VECTA2100","price":344.5,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/a-2100-100_alkaline_phosphatase_avidin_d-jpg.webp?v=1753117840"},{"product_id":"avidin-dn","title":"Avidin DN","description":"\u003cp\u003eAvidin DN, 1 mg, is isolated by an affinity chromatographic system that results in a form of avidin which, like Avidin DH, exhibits very low non-specific binding to nucleic acids. Although the reasons for this property are not fully understood, this feature can be exploited when using biotin-avidin detection systems with biotin-labeled DNA or RNA probes for in situ or nitrocellulose hybridization techniques.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53617461002531,"sku":"VECTA3100","price":300.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/a-3100-1_avidin_dn_1.webp?v=1753117844"},{"product_id":"biotinylated-protein-a","title":"Biotinylated Protein A","description":"\u003cp\u003eProtein A, Biotinylated (S. Aureus), 1 mg, is a conjugated form of the 41 kDa protein that binds the Fc portion of most immunoglobulins, including IgA, IgM, and subclasses of IgG. This interaction is neither species- nor isotype-specific. Biotinylated Protein A is useful to detect or localize immunoglobulins on cell surfaces or in tissue sections through its combination with an appropriate avidin or streptavidin conjugate for brightfield, fluorescence, or electron microscopy. It can also be used to detect positive clones in monoclonal antibody production and is used in many other systems as a substitute for a biotinylated second antibody. In some applications, Biotinylated Protein A may bind to endogenous immunoglobulin in the tissue producing background. In others, it may not bind to the species or class of primary antibody being employed. As such, for routine IHC staining, biotinylated anti-immunoglobulins are recommended.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53617470505251,"sku":"VECTB2001","price":274.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/b-2001-1_protein_a_biotinylated.webp?v=1753117922"},{"product_id":"cy3-streptavidin","title":"Cy3 Streptavidin","description":"\u003cp\u003eStreptavidin, CY3, 1 mg, can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin\/avidin or biotin\/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. Excitation: 550 nm; Emission: 570 nm; Color: Red.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618265522467,"sku":"VECTSA1300","price":384.3,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/cy3strept5-1000-jpg.webp?v=1753122435"},{"product_id":"cy5-streptavidin","title":"CY5 Streptavidin","description":"\u003cp\u003eStreptavidin, CY5, 1 mg, can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin\/avidin or biotin\/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. Excitation: 649 nm; Emission: 670 nm; Color: Far Red.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618265555235,"sku":"VECTSA1500","price":384.3,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/cy5strept3-1000-jpg.webp?v=1753122439"},{"product_id":"streptavidin-1-mg-1","title":"Streptavidin, 1 mg","description":"\u003cp\u003eStreptavidin, 1 mg. Vector Laboratories Streptavidin is isolated from Streptomyces avidinii by a specific affinity system, which results in a homogeneous, ultrapure streptavidin with very low non-specific binding characteristics. Unconjugated streptavidin is recommended for use in solid-phase assays and immunohistochemical applications when the technique of sequential addition of reagents (sandwich technique) is employed. This reagent has also been effectively employed in subtractive hybridization procedures.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618265588003,"sku":"VECTSA5000","price":274.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/sa-5000-1_streptavidin-jpg.webp?v=1753122442"},{"product_id":"fluorescein-streptavidin","title":"Fluorescein Streptavidin","description":"\u003cp\u003eStreptavidin, Fluorescein, 1 mg, can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified, possess very low non-specific binding, and have extremely high affinity for biotin. Easily achieve amplification of fluorescent signals with our biotinylated secondary antibodies and highly purified fluorochrome-labeled streptavidin or avidin. Using biotin\/avidin or biotin\/streptavidin detection results in an additional amplification over a directly conjugated secondary antibody. Excitation max: 495 nm; Emission max: ~ 515 nm. This product has an optimal fluorochrome to protein ratio and is a reagent we recommend for routine immunofluorescence applications. This product can provide greater sensitivity and lower background staining than conventional primary and secondary fluorescent techniques.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618265719075,"sku":"VECTSA5001","price":343.9,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/sa-5001-1_streptavidin_fluorescein-jpg.webp?v=1753122446"},{"product_id":"horseradish-peroxidase-streptavidin","title":"Horseradish Peroxidase Streptavidin","description":"\u003cp\u003eStreptavidin, Peroxidase (Concentrate, for IHC), 1 mg, is a powerful tool to detect or purify biotinylated proteins, nucleic acids, and other macromolecules. This product is produced by a coupling procedure that preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears. This product is also available in a Ready-to-Use format (SA-5704). For horseradish peroxidase streptavidin (Sav-HRP) preparation that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots (Cat. No. SA-5014).\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618265817379,"sku":"VECTSA5004","price":322.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/sa-5004-1._streptavidin_peroxidase_ihc-jpg.webp?v=1753122450"},{"product_id":"texas-red-streptavidin","title":"Texas Red Streptavidin","description":"\u003cp\u003eStreptavidin, Texas Red, 1 mg. Texas Red Streptavidin is a highly fluorescent conjugate of streptavidin and sulforhodamine 101. It can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Features: Highly purified and possesses very low non-specific binding properties; Has a high fluorochrome to protein ratio; Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining. Excitation maximum: 595 nm; Emission maximum: 615 nm; Color: Red.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618265882915,"sku":"VECTSA5006","price":361.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/sa-5006-1_streptavidin_texas_red-jpg.webp?v=1753122453"},{"product_id":"horseradish-peroxidase-streptavidin-elisa-grade","title":"Horseradish Peroxidase Streptavidin, ELISA Grade","description":"\u003cp\u003eStreptavidin, Peroxidase, ELISA Grade (Concentrate, for ELISAs and Blots), 1 mg. Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules. Vector Laboratories enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates. This preparation (HRP-Sav; Cat. No. SA-5014) is optimized for use in solid-phase assays, such as ELISAs and blotting applications. For a preparation that is optimized for use in tissue\/cell staining applications, see Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC\/ICC (Cat. No. SA-5004).\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618266079523,"sku":"VECTSA5014","price":323.3,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/sa-5014-1_streptavidin_peroxidase_elisa_blots-jpg.webp?v=1753122463"},{"product_id":"alkaline-phosphatase-streptavidin","title":"Alkaline Phosphatase Streptavidin","description":"\u003cp\u003eStreptavidin, Alkaline Phosphatase, 1 ml. Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules. Vector Laboratories enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates. These enzyme conjugates are suitable for use in solid-phase assays, tissue\/cell staining systems, and blotting applications.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618266145059,"sku":"VECTSA5100","price":396.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/sa-5100-1_streptavidin_ap-jpg.webp?v=1753122466"},{"product_id":"dylight-488-streptavidin","title":"DyLight 488 Streptavidin","description":"\u003cp\u003eStreptavidin, DyLight 488, 1 mg. DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence. Excitation maximum: 493 nm; Emission maximum: 518 nm; Color: Green.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618266177827,"sku":"VECTSA5488","price":382.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/ee12_13-jpg.webp?v=1753122469"},{"product_id":"dylight-549-streptavidin","title":"DyLight 549 Streptavidin","description":"\u003cp\u003eStreptavidin, DyLight 549, 1 mg, can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Features: Highly purified and possess very low non-specific binding properties, Extremely high affinity for biotin. Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence. Excitation at 556 nm; Emission at 571 nm; Color: Orange.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618266243363,"sku":"VECTSA5549","price":382.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/11gfaprb549s_9-jpg.webp?v=1753122473"},{"product_id":"dylight-594-streptavidin","title":"DyLight 594 Streptavidin","description":"\u003cp\u003eStreptavidin, DyLight 594, 1 mg, can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Features: Highly purified and possess very low non-specific binding properties, Extremely high affinity for biotin. Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence. Excitation at 592 nm; Emission at 617 nm; Color: Red.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618266308899,"sku":"VECTSA5594","price":385.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/ee16_13-jpg.webp?v=1753122477"},{"product_id":"dylight-649-streptavidin","title":"DyLight 649 Streptavidin","description":"\u003cp\u003eStreptavidin, DyLight 649, 1 mg, can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Features: Highly purified and possess very low non-specific binding properties, Extremely high affinity for biotin. Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence. Excitation at 655 nm; Emission at 670 nm; Color: Far Red.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618266374435,"sku":"VECTSA5649","price":382.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/sa-5649-1_streptavidin_dylight649-jpg.webp?v=1753122480"},{"product_id":"r-t-u-horseradish-peroxidase-streptavidin","title":"R.T.U. Horseradish Peroxidase Streptavidin","description":"\u003cp\u003eStreptavidin, Peroxidase, R.T.U., 100 ml, is a powerful tool to detect or purify biotinylated proteins, nucleic acids, and other macromolecules. This Ready-To-Use (R.T.U.) version is produced by Vector Laboratories, using a coupling procedure that preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears. This product is supplied in a bottle fitted with a drop dispenser top. For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product: Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots.\u003c\/p\u003e","brand":"Vector Laboratories","offers":[{"title":"Default Title","offer_id":53618266439971,"sku":"VECTSA5704","price":331.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/sa-5704-100_streptavidin_peroxidase_rtu-jpg.webp?v=1753122484"}],"url":"https:\/\/chromspec.com\/collections\/15575-avidin-and-biotinylated-reagents.oembed?page=2","provider":"Chromatographic Specialties Inc.","version":"1.0","type":"link"}