**Nucleosome, Recombinant Human, H3K27ac dNuc Biotinylated, Ship Product on Dry Ice

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EP16036520
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  • Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains acetyl-lysine at position 27. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom [1], is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.
  • *H3K27ac also has a Cys to Ala substitution at position 110
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20UG
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DRYICE - Product Ships with Dry Ice