HOOK™ Peptide Coupling Kit (Sulfhydryl reactive) with OneQuant™ HyperCarrier™, 5 Reactions

SKU:
GB786074
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Protocol

  • The HOOK Sulfhydryl Reactive Peptide Coupling Kit is designed for the coupling of peptides to carrier proteins, utilizing a sulfhydryl group in the peptide.
  • This kit exploits the chemical crosslinker sulfoSMCC (Sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) to couple peptides through their sulfhydryl groups, found on cysteine side chains, to the primary amines on the carrier proteins.
  • The Nhydroxysuccinimide (NHS) ester in sulfoSMCC reacts with primary amines to form covalent amide bonds and the maleimide group reacts with sulfhydryl groups to form stable thioether bonds.
Sales Unit:
KIT
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ROOMTEMP - Ships at Room Temperature