A simple method for fractionation of cytoplasmic and nuclear proteins from cells and tissues.
The fractionation method is based on gentle lysis of sample followed by centrifugation to collect and separate nuclei from the rest of cytoplasmic proteins.
For proteomic as well as expression and transport studies.
Supplied with a proprietary chaotropic extraction buffer for solubilization of both nuclear proteins and cytoplasmic proteins for 2D analysis
Suitable for sample preparation for 2D gel electrophoresis and other applications