GST Binding/ Wash Buffer (PBS + 1% Triton® X-100), 100 mL

SKU:
GB786540
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Protocol

  • The purification of His-tagged proteins is carried out by immobilized metal affinity chromatography (IMAC) with nickel being the preferred metal ion. Elutions of tag proteins is achieved with imidazole.
  • The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads.
  • The iminodiacetate binds divalent nickel ion with a capacity of 20-40 moles Ni2+/mL resin.
Sales Unit:
100ML
Additional_Shipping:
ROOMTEMP - Ships at Room Temperature