S100A4, Human, ELISA kit, 1 x 96 det.

SKU:
HYBHK37701
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Description

Product Manual

  • The Human S100A4 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.
  • The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay.
  • Samples and standards are incubated in microtiter wells coated with antibodies recognizing Human S100A4.
  • Biotinylated tracer antibody will bind to the captured Human S100A4.
  • Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody.
  • Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB).
  • The enzyme reaction is stopped by the addition of oxalic acid.
  • The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Human S100A4 standards (log).
  • The Human S100A4 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve
Additional Information
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KIT
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