L-FABP, Human, ELISA kit, 2 x 96 det.

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HYBHK40402
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  • Fatty acid-binding proteins (FABPs) are a class of cytoplasmic proteins that bind long chain fatty acids. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. Liver-type fatty acid binding protein (L-FABP, FABP1) is predominantly expressed in liver. The L-FABP protein is derived from the human FABP1 gene. L-FABP is a sensitive marker for cell damage of liver cells in vitro and in vivo. L-FABP is also a marker for rapid hepatocyte lysis in vitro (as for example in toxicology assays) and for detection of liver damage during and after transplantation. Serum/plasma and urine of healthy individuals contains approximately 12 ng/ml and 16 ng/ml L-FABP, respectively.
  • The human L-FABP ELISA has been developed for the quantitative measurement of natural and recombinant human L-FABP in cell culture medium, serum, plasma and urine. In serum or plasma samples human L-FABP can be measured accurately if serum or plasma samples are diluted at least 20 times.
  • The cross reactivity with Human H-FABP and Human I-FABP was negative.
  • Optional use: Cytotoxicity protocol
  • Culture 100 µl of 1.105 HUH-7 cells per ml in 96-wells plate.
  • T=0: Add 100 µl of 2x test chemicals to 96-wells plate.
  • Incubate 37 °C, 5% CO2.
  • T=24: measure L-FABP in supernatant.
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