SFD-10HT, 5 mL

SFD-10HT, 5 mL

SKU:
MGSF80005
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Description

Depletes DNA Fragments <10 kb

The First Magnetic bead-based reagent for efficient removal of short DNA Fragments 

Short Fragment Depletor-10 High Throughput (SFD-10HT) reagent is a bead-based reagent used for size selection and purification of high molecular weight DNA in long read sequencing. It can significantly improve the mean read length by near depletion of DNA fragments below 10 kb. This kit is suitable for amplicons, sheared/fragmented DNA and genomic DNA. SFD-10HT working concentration range is 0.5-150 ng/µL.

Applications

  • High Molecular Weight DNA: purification and concentration 
  • Sheared/fragmented DNA: removal of fragments <10 kb 
  • Library cleanup and rapid size selection >10 kb 

Benefits

  • Enhancement of DNA mean read length by removal of short fragments (<10 kb) prior to library preparation 
  • During SFD-10HT size selection and short fragment depletion, DNA is purified, which helps remove contaminants that may be carried over from the extraction process.
  • Concentrates dilute HMW DNA samples 
  • Tolerant to a wide range of DNA input range (0.5-150 ng/µL) 
  • Applicable in a wide variety of sample types: HMW DNA, fragmented DNA, sheared DNA, and PCR amplicons/libraries 
  • Bead-based - no centrifugation  
  • Efficient scale-up and flexibility - amenable to automation 
  • Reproducible results 
  • High recovery efficiency (50-90%) depending on quality and concentration of input DNA 
  • Saves time - protocol less than 65 minutes
Additional Information
Sales Unit:
5ML
Additional_Shipping:
ROOMTEMP - Ships at Room Temperature