{"product_id":"rnase-a-dnase-free-rnase-a-dnase-free-endoribonuclease-bovine-pancreas-cas-9001-99-4-2-x-50-mg","title":"RNase A (DNase free), RNase A (DNase free), Endoribonuclease, Bovine Pancreas, CAS # 9001-99-4, 2 x 50 mg","description":"\u003cp class=\"richp\"\u003eRNase A is an endoribonuclease that attacks at the 3'-phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with ssRNA.\u003c\/p\u003e\u003cp class=\"richp\"\u003eRNase A is free of detectable DNase and protease activity, a heat treatment of the enzyme is not necessary before use.\u003c\/p\u003e\u003cp class=\"richp\" sugg=\"true\"\u003e\u003cb\u003eShipping:  \u003c\/b\u003eShipped at ambient temperature\u003c\/p\u003e\u003cp class=\"richp\" sugg=\"true\"\u003e\u003cb\u003eStorage Conditions:\u003c\/b\u003e Store at 2-8 °C.  Stock solutions in TE buffer should be aliquoted and stored at -20 °C\u003c\/p\u003e\u003cp class=\"richp\"\u003e\u003cb\u003eShelf Life\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp class=\"richp\"\u003e\u003cb\u003eMolecular Weight:\u003c\/b\u003e 13.7 kDa (monomer)\u003c\/p\u003e\u003cp class=\"richp\"\u003e\u003cb\u003eCAS#:\u003c\/b\u003e 9001-99-4\u003c\/p\u003e\u003cp class=\"richp\" sugg=\"true\"\u003e\u003cb\u003eEC number:  \u003c\/b\u003e232-646-6\u003c\/p\u003e\u003cp class=\"richp\"\u003e\u003cb\u003ePurity: \u003c\/b\u003e≥ 90 % (ion exchange chromatography), salt free, chromatographically homogeneous lyophilisate\u003c\/p\u003e\u003cp class=\"richp\"\u003e\u003cb\u003eForm: \u003c\/b\u003edry powder\u003c\/p\u003e\u003cp class=\"richp\" sugg=\"true\"\u003e\u003cb\u003eActivity:\u003c\/b\u003e  ≥ 80 Kunitz units\/mg\u003c\/p\u003e\u003cp class=\"richp\"\u003eUnit definition: 1 Kunitz unit is that amount of activity which is capable of causing within 1 minute a decrease in absorbance at\u003c\/p\u003e\u003cp class=\"richp\"\u003e300 nm equivalent to the maximum possible change in a 0.05 % solution of yeast RNA at 25 °C, pH 5.0.\u003c\/p\u003e\u003cp class=\"richp\"\u003e\u003cb\u003eApplications:\u003c\/b\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003ePlasmid and genomic DNA preparation\u003c\/li\u003e\n\u003cli\u003eRemoval of RNA from recombinant protein preparations\u003c\/li\u003e\n\u003cli\u003eRibonuclease protection assays\u003c\/li\u003e\n\u003cli\u003eMapping single-base mutations in DNA or RNA\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp class=\"richp\" sugg=\"true\"\u003e\u003cb\u003eReaction conditions:  \u003c\/b\u003e\u003c\/p\u003e\u003cp class=\"richp\" sugg=\"true\"\u003eWorking concentration: 1 - 100 μg\/ml (depending on application)\u003c\/p\u003e\u003cp class=\"richp\"\u003eThe enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase cleaves ss and dsRNA as well as the RNA strand in RNA-DNA hybrids. At NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves ssRNA.\u003c\/p\u003e\u003cp class=\"richp\" sugg=\"true\"\u003e\u003cb\u003eStability:  \u003c\/b\u003e\u003c\/p\u003e\u003cp class=\"richp\" sugg=\"true\"\u003eRNase A is an extremely stable enzyme, remarkable resistant to heating. It renatures easily after treatment with most denaturing agents.\u003c\/p\u003e\u003cp class=\"richp\" sugg=\"true\"\u003e\u003cb\u003eInactivation:  \u003c\/b\u003e\u003c\/p\u003e\u003cp class=\"richp\" sugg=\"true\"\u003eRibonuclease inhibitor, Vanadyl-ribonucleoside complexes, arabinonucleosides, Zn2+, Cu2+, penicillin, Vitamin B12, SDS, DEPC, 4 M guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol. Most polyanions show some inhibitory effect. Inactivated by phenol\/chloroform extraction.\u003c\/p\u003e\u003cp class=\"richp\"\u003eIsoelectric point (pI):9.6\u003c\/p\u003e\u003cp class=\"richp\" sugg=\"true\"\u003e\u003cb\u003eOptimal pH:  \u003c\/b\u003e7.0 (activity range 6 - 10)\u003c\/p\u003e","brand":"Jena Bioscience","offers":[{"title":"Default Title","offer_id":55609654378787,"sku":"JBEN179S","price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0926\/3870\/8003\/files\/Jena_default_product_image_2048x2048pxl_47510c1b-faa4-4030-ac56-09bd54e18712.jpg?v=1789668386","url":"https:\/\/chromspec.com\/products\/rnase-a-dnase-free-rnase-a-dnase-free-endoribonuclease-bovine-pancreas-cas-9001-99-4-2-x-50-mg","provider":"Chromatographic Specialties Inc.","version":"1.0","type":"link"}