HOT FIREPol® Multiplex qPCR Mix, 20 mL (5000 rxn)

SKU:
SOL080100020
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Description

Product Details

Datasheet

  • HOT FIREPol® Multiplex qPCR Mix is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes, and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, and MgCl2.
  • HOT FIREPol® Multiplex qPCR Mix is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity.
  • HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
  • Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction.
  • Probe-based qPCR master mix that has been optimized for highly sensitive and accurate quantification of up to 4 targets in a single reaction. This master mix was developed for TaqMan® probes but is also suitable for other hydrolysis probe types
  • Analyze 1-4 targets in 1 reaction
  • High specificity and sensitivity
  • Robust amplification of GC-rich targets
  • Contains dUTP to prevent cross-contamination when used in combination with UNG
Additional Information
Sales Unit:
20ML
Additional_Shipping:
ROOMTEMP - Ships at Room Temperature