HOT FIREPol® Probe qPCR Mix Plus (no ROX), 20 mL (5000 rxn)

SKU:
SOL081500020
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Description

Product Details

Datasheet

  • HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe.
  • The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity.
  • HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
  • Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
  • Cost-effective real-time qPCR master mix for probe-based qPCR assays.
  • This master mix has been developed for TaqMan® probes but is suitable for other hydrolysis probe types as well.
  • Suitable for singleplex and duplex assays
  • High specificity and sensitivity
  • Reaction set-up and shipment without dry ice
Additional Information
Sales Unit:
20ML
Additional_Shipping:
ROOMTEMP - Ships at Room Temperature