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Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound, 2 ml, is an agarose-conjugated form of ECL. This lectin consists of two different subunits of approximately 28 kDa and 26 kDa, and the carbohydrate structure to which it binds is frequently found in membrane and serum glycoproteins of mammalian origin. Covalent attachment of ECL to the agarose beads (using a hydrophilic spacer arm) preserves lectin activity and minimizes conformational changes that might result in non-specific or hydrophobic interactions. This also ensures that conjugated proteins are not leached off the beads by Tris or other routinely used buffers. Agarose-conjugated ECL is supplied as a 1:1 suspension in buffer and comprises a matrix of heat-stable, crosslinked 4% agarose beads that is stable in solutions at pH 3–11 as well as many organic solvents. Because no residual charge is present after conjugation, non-specific binding to the matrix is minimized.
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Vector Laboratories
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Vector Laboratories
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Vector Laboratories
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Vector Laboratories
