Agarose Streptavidin

Vector Laboratories

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VECTSA5010

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Product Details

Streptavidin, Agarose, 2 ml, is prepared by conjugating streptavidin to heat-stable, crosslinked 4% agarose gel beads. To ensure minimal steric interference and low non-specific binding, streptavidin is conjugated through a hydrophilic spacer arm. The Vector Laboratories procedure for coupling streptavidin to agarose preserves the biotin binding activity of the streptavidin. Unlike cyanogen bromide coupling, this procedure does not produce conjugates that can be leached from the gel with solutes, such as Tris buffer. It also does not generate charged groups on the gel that can bind proteins non-specifically. Protein concentration is 1 mg streptavidin per ml settled agarose beads. The product is supplied as a 1:1 suspension in buffer. The binding capacity is approximately 1 mole biotinylated HRP per mole of streptavidin. This product can be used to separate biotinylated macromolecules from unbiotinylated materials or for solid-phase binding assays.