Acclaim™ Mixed Mode WAX-1 HPLC Column, 50 x 3.0 mm, 3 µm

When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific™ Acclaim™ Mixed-Mode WAX-1 LC columns. The silica-based stationary phase features a hydrophobic alkyl chain with an ionizable terminus that provides both hydrophobic reversed-phase and weak anion-exchange properties. This unique chemistry produces a multimode separation mechanism for simultaneous separation of acidic, basic, and neutral molecules. The selectivity is fully adjustable through changes to ionic strength, pH, or organic solvent content.

Ideal Selectivity for Separating Anionic Molecules

• Multi-mode retention mechanism: reversed-phase, weak anion-exchange, and HILIC modes
• Differentiation of molecules with similar hydrophobicities, such as hydrophilic organic acids
• Selectivity orthogonal to reversed-phase columns
• Excellent column efficiency and peak symmetry
• Stable between pH 2.5 and 7.5
• Available in 3 and 5 μm particle sizes with an average pore diameter of 120 Å

Specifications:

Column Format	Analytical Column
Column Type	Mixed Mode
Endcapped	Proprietary
For Use With	HILIC
Maximum Pressure	4500 psi (310 bar)
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Stationary Phase	Hydrophobic Alkyl Chain with an Ionizable Terminus
Surface Area	300 m²/g
Maximum Temperature	50°C
USP Type	L78
Diameter (Metric)	3 mm
Length (Metric)	50 mm
Particle Size	3 μm
Phase	Reversed Phase, Ion Exchange, HILIC
Pore Size	120 Å
Product Line	Acclaim
pH Range	2.5 to 7.5