***Biotin-14-dATP, 5 x 200 µL

Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N6 position of adenine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dATP is recommended whose Biotin moiety is attached to the N7-Deaza position of adenine via a 11-atom linker. Recommended Biotin-14-dATP/dATP ratio for Nick Translation: 50% Biotin-14-dATP/50% dATP.

• Alternative Names:  Biotin-14-N6-(6-Aminohexyl)-dATP, Triethylammonium salt, Bio-14-dATP
• MW:  Theoretical MW: 929.81 g/mol (free acid); Detected MW: 929.27 g/mol (free acid)
• Purity:  ≥ 95% (HPLC)
• Formula:  C32H54N9O15P3S
• Storage:  store at -20 °C
• Expiration Date:  12 months from date of receipt.
• Hazard Information:  Non-Toxic
• Disclaimer:  For research use only