HOT SolisAcura™ Cassette-based Genotyping Mix, 1 mL (200 rxn)

HOT SolisAcura™ Cassette-based Genotyping Mix

Accurate, inhibitor tolerant and high-specificity cassette-based genotyping mix for precise bi-allelic SNP detection.

Features and Benefits:

• Superior mismatch discrimination at the 3’ end of primers
• Universal cassette-based reporter system
• Accurate genotype call with high rate of confidence
• Reliable and efficient differenciation of clusters
• Superb performance with crude samples
• Tolerant to common PCR inhibitors

HOT SolisAcura™ Cassette-Based Genotyping Mix (ROX) is based on genetically modified HOT SolisAcura™ Exo(-) DNA Polymerase, giving the master mix a feature of detecting misaligned nucleotide at the 3’ ends of primer, resulting in superior performance in SNP detection and allele discrimination. It has 10-15x higher fidelity compared to wild-type Taq polymerase, which comes from choosing nucleotides more accurately. Additionally, there is no 3′→5′ exonuclease (proofreading) activity with this enzyme.

The novel polymerase is also engineered to have faster synthesis rates and is inherently inhibitor tolerant, ensuring excellent performance with crude or challenging samples. It comes with chemical hot-start that improves the specificity and accuracy of the reaction, minimizes mispriming and extension from non-specifically annealed primers and primer-dimers.

This enzyme is suitable for SNP detection via allele-specific PCR, using bi-allelic discrimination that is achieved with a universal reverse primer and two competitively binding allele-specific forward primers, each with a unique tail sequence that corresponds to either of two universal FRET cassettes. HOT SolisAcura™ Cassette-Based Genotyping Mix (ROX) is also compatible with KASP™, PACE® and Amplifluor® marker assays.

HOT SolisAcura™ Cassette-Based Genotyping Mix (ROX) is room temperature stable for up to 1 month due to the use of Stability TAG – Solis BioDyne’s proprietary and patented polypeptide stabilization technology that is incorporated into the enzyme, enabling environmentally friendly ambient temperature shipping [1]. Room temperature stability also enables maximum convenience allowing reactions to be set up without ice at room temperature.