Bioconjugation

Conjugate with confidence

Leverage bioconjugation to expand your molecular toolbox and create the perfect match for your research needs. SoluLINK® bioconjugation technology is engineered for high-performance conjugation of all classes of biomolecules, including antibodies, proteins, peptides, oligonucleotides, carbohydrates, drugs, and surfaces. The result is reliable, quantifiable labeling delivering ready-to-use conjugates at high yield.

Webinar: An Introduction to Bioconjugation

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Bioconjugation Resource Guide: Methodology to Linking Technology

This guide provides an overview of bioconjugation, applications and uses, as well as tools for successful bioconjugation.

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Quantitative and Reproducible Bioconjugation with SoluLINK Technology

Quantitative and Reproducible Bioconjugation with SoluLINK Technology

Explore the bioconjugation workflow and considerations for selecting a conjugation strategy.

How it works
SoluLINK technology uses an innovative linker-based strategy to chemically join two molecules to form a single hybrid with a novel function.
The chemistry is based on the reaction of an aromatic hydrazine (HyNic) with an aromatic aldehyde (4FB), which forms a stable bis-aryl hydrazone bond.

This UV-traceable chromophore helps ensure experimental success with direct quantification to: 

  • Determine of the number of linkers incorporated on each biomolecule prior to conjugation
  • Monitor and record the conjugation reaction in real time
  • Measure of the number of linkages formed in the conjugate and the precise number of biomolecules or ligands attached
  • Conjugation Kits

    From conjugation to purification, Vector's fully integrated kits ensure the generation of conjugates at high yield, with direct quantification to confirm experimental success.


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  • Magnetic Beads & Agarose

    NanoLINK® and MagnaLINK® straptavidin magnetic beads, as well as streptavidin agarose, provide up to 15-times greater biotin binding capacity than other commercially available products.



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  • Biotin and Digoxigenin Labeling Reagents

    ChromaLINK® Biotin and Digoxigenin products enable accurate, controlled incorporation of labels on biomolecules, without the need to carry out cumbersome and time-consuming HABA/avidin and fluoro-reporter assays.

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Looking to scale your conjugation with maximum performance?

Vector's extensive portfolio and bioconjugation experts can confirm successful scale-up of conjugation for your analysis.

  • Conjugation Linkers

    To meet your needs for scale-up conjugation, amine-reactive NHS ester and thiol-reactive maleimide versions of HyNic, 4FB, Biotin, and Digoxigenin linkers are available as stand-alone options.

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  • Bioconjugation Ancillary Products

    Stand-alone linkers and reagents for scale-up manufacturing.



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  • Custom Services

    Vector's dedicated team of bioconjugation experts are available to suggest conjugation and purification strategies for maximum performance in your application.

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Questions?

Check out Vector’s FAQs to learn more.

References:

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4. Kaur M. et al. 2019. Induction and Therapeutic Targeting of Human NPM1c+ Myeloid Leukemia in the Presence of Autologous Immune System in MiceThe Journal of Immunology
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10. Klaesson A. et al. 2018. Improved efficiency of in situ protein analysis by proximity ligation using UnFold probesScientific Reports
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12. Thinn AMM. et al. 2018. The membrane-distal regions of integrin α cytoplasmic domains contribute differently to integrin inside-out activationScientific Reports
13. Ambrosetti E. et al. 2017. Quantification of Circulating Cancer Biomarkers via Sensitive Topographic Measurements on Single Binder Nanoarrays. ACS Omega
14. Sehlin D. et al. 2017. Pharmacokinetics, biodistribution and brain retention of a bispecific antibody-based PET radioligand for imaging of amyloid-ßScientific Reports
15. Han BG. et al. 2016. Long shelf-life streptavidin support-films suitable for electron microscopy of biological macromolecules. Journal of Structural Biology
16. Sehlin D. et al. 2016. Antibody-based PET imaging of amyloid beta in mouse models of Alzheimer’s disease. Nature Communications
17. Gu Urban G. et al. 2015. Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome. Scientific Reports
18. Volpetti F. et al. 2015. A Microfluidic Platform for High-Throughput Multiplexed Protein Quantitation. PLOS ONE
19. Hammond M. et al. 2014. Sensitive detection of aggregated prion protein via proximity ligation. Prion
20. Torres AJ. et al. 2013. Functional single-cell analysis of T-cell activation by supported lipid bilayer-tethered ligands on arrays of nanowells. Lab on a Chip
21. Malviya G. et al. 2009. Radiolabeled Humanized Anti-CD3 Monoclonal Antibody Visilizumab for Imaging Human T-Lymphocytes. Journal of Nuclear Medicine
22. Liu G. et al. 2007. A novel pretargeting method for measuring antibody internalization in tumor cells. Cancer Biotherapy & Radiopharmaceuticals
23. Dirksen A. & Dawson PE. 2008. Rapid Oxime and Hydrazone Ligations with Aromatic Aldehydes for Biomolecular Labeling. Bioconjugate Chemistry
24. Dirksen A. et al. 2006. Nucleophilic Catalysis of Hydrazone Formation and Transimination: Implications for Dynamic Covalent Chemistry. Journal of the American Chemical Society