Mirus Bio – Transfection
The Transfection Team
TransIT-X2® Dynamic Delivery System
A dynamic transfection system for the delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components.
A versatile transfection reagent that is the solution for hard-to-transfection and common cell types.
Proven and reliable, TransIT-X2® allows you to avoid guesswork and get it right the first time.
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Peak performance in a broad range of cell types
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Suitable for multiple applications
- Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 component
TransIT®-LT1 Transfection Reagent
A broad spectrum, low toxicity, DNA transfection reagent.
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Broad Spectrum DNA Delivery – Utilize one transfection reagent and protocol for a variety of cells
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Low Cellular Toxicity – Maintain cell density and reduce experimental biases.
- High Efficiency Delivery – Achieve expression in a large population of cells for
- Deliver Single or Multiple Plasmids – Suitable for applications such as virus production
TransIT®-2020 Transfection Reagent
A high performance, animal-free, broad spectrum DNA transfection reagent.
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Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
and primary cells
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Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity
- Animal Origin Free – High performance with maximum compatibility
| Product Number | Description | Pkg Size |
|---|---|---|
| MIR5404 | TransIT®-2020 Transfection Reagent | 0.4 mL |
| MIR5400 | TransIT®-2020 Transfection Reagent | 1.0 mL |
| MIR5405 | TransIT®-2020 Transfection Reagent | 5 × 1.0 mL |
| MIR5406 | TransIT®-2020 Transfection Reagent | 10 × 1.0 mL |
TransIT®-mRNA Transfection Kit
A high efficiency, low toxicity transfection reagent for large RNA and CRISPR guide RNA.
Each Kit is supplied with the TransIT®-mRNA Transfection Reagent and the mRNA Boost Reagent.
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Low Cellular Toxicity – Maintain cell density and reduce experimental biases
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High Efficiency Delivery – Achieve RNA delivery in a large population of cells to ensure experimental success
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Serum Compatible – Perform transfections in the presence of serum which eliminates the need for a media change
and maintains cellular health
- Deliver Various Sizes of RNA – Ideal for specialized applications, such as virus production, protein expression and
CRISPR genome editing.
TransIT®-TKO Transfection Reagent
A high efficiency, low toxicity, siRNA and plasmid DNA transfection reagent for mammalian cells.
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Broad Spectrum siRNA and Plasmid DNA Delivery – Utilize one transfection reagent and protocol for a wide
variety of cells
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Low Cellular Toxicity – Maintain cell density and reduce experimental biases
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Reproducible Results – Obtain consistent, targeted gene knockdowns from day to day
- High Knockdown Efficiency – Achieve optimal gene silencing in a large percentage of cells to ensure
experimental success.
TransIT®-siQUEST Transfection Reagent
A high efficiency, low toxicity, siRNA transfection reagent for mammalian cells.
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Broad Spectrum siRNA Delivery – Utilize one transfection reagent and protocol for a wide variety of cells
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Low Cellular Toxicity – Maintain cell density and reduce experimental biases
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Reproducible Results – Obtain consistent, targeted gene knockdowns from day to day
- High Knockdown Efficiency – Achieve optimal gene silencing in a large percentage of cells to ensure
experimental success
TransIT®-Oligo
A high efficiency, low toxicity, transfection reagent optimized for oligonucleotide delivery into a wide range of cell types.
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Unique Formulation – Maximize transfection performance of oligonucleotides into a wide range of cells
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Low Cellular Toxicity – Maintain cell density and reduce experimental biases
- High Efficiency Delivery – Achieve high transfection efficiency in cells to ensure experimental success
| Product Number | Description | Pkg Size |
|---|---|---|
| MIR2164 | TransIT®-Oligo Transfection Reagent | 0.4 mL |
| MIR2160 | TransIT®-Oligo Transfection Reagent | 1.0 mL |
| MIR2165 | TransIT®-Oligo Transfection Reagent | 5 × 1.0 mL |
| MIR2166 | TransIT®-Oligo Transfection Reagent | 10 × 1.0 mL |
Electroporation with Ingenio®
Mirus Bio offers a full range of electroporation offerings, including; Ingenio® Electroporation Solution, electroporation cuvettes, and the new Ingenio® EZporator® Electroporation System. These products are designed to high efficiency delivery to hard-to-transfect cells without the cost or complication of other systems.
Ingenio® EZporator®
The Ingenio® EZporator® Electroporation System is an easy-to-use electroporation system for mammalian cells.
• Easily optimize electroporation parameters for each cell type
• Every system comes with a complimentary Ingenio® Electroporation Kit
| Product Number | Description |
|---|---|
| MIR51000 | Ingenio® EZporator® |
Ingenio® Electroporation Solution
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Compatible with multiple cell types and devices.
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Use your existing system including the Ingenio® EZporator®, Nucleofector®, Bio-Rad® or Harvard BTX®
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Reduce cost when using the Lonza Nucleofector® without sacrificing performance
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High efficiency electroporation of hard to transfect cells, including primary cells and stem cells
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Long shelf life - One year shelf life allows maximum usage for your experiments
- Buy only what you need - Available as solution alone or as part of a kit with cuvettes and cell droppers
| Product Number | Description | Pkg Size |
|---|---|---|
| MIR50125 | Ingenio Cell Droppers | 50 |
| MIR50121 | Ingenio 0.2 cm Cuvettes | 50 |
| MIR50123 | Ingenio 0.4 cm Cuvettes | 50 |
| MIR50112 | Ingenio Kit-Amaxa, 0.2 cm Cuvettes/Droppers | 25 rxn |
| MIR50115 | Ingenio Kit-Amaxa, 0.2 cm Cuvettes/Droppers | 50 rxn |
| MIR50118 | Ingenio Kit-Amaxa, 0.2 cm Cuvettes/Droppers | 100 rxn |
| MIR50113 | Ingenio Kit-Other, 0.4 cm Cuvettes/Droppers | 25 rxn |
| MIR50116 | Ingenio Kit-Other, 0.4 cm Cuvettes/Droppers | 50 rxn |
| MIR50119 | Ingenio Kit-Other, 0.4 cm Cuvettes/Droppers | 100 rxn |
The Cell and Gene Therapy Virus Production
TransIT-VirusGEN® Transfection Reagent
Proven Nucleic Acid Delivery for Large-Scale Virus Production
Designed to enhance delivery of packaging and transfer plasmids to adherent and suspension HEK 293 cell types to increase recombinant adeno-associated virus (AAV) and lentivirus production.
Key benefits of TransIT-VirusGEN® Transfection Reagent include:
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Performance – Efficient DNA delivery for production of high-titer viral vectors
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Scalability – Efficient production from < 1 ml to large scale cultures
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Flexibility – Compatible with different recombinant virus and cell culture systems
- Animal Origin Free – Fully synthetic transfection reagent formulation
TransIT-VirusGEN® is ideal for academic research and biopharmaceutical discovery. Titers can be further increased with optimized enhancers included in the VirusGEN® AAV Transfection Kit and VirusGEN® LV Transfection Kit. For large-scale production of viral vectors, Mirus recommends TransIT-VirusGEN® GMP.
| Product Number | Description | Pkg Size |
|---|---|---|
| MIR6703 | TransIT-VirusGEN® Transfection Reagent | 0.3 mL |
| MIR6704 | TransIT-VirusGEN® Transfection Reagent | 0.75 mL |
| MIR6700 | TransIT-VirusGEN® Transfection Reagent | 1.5 mL |
| MIR6705 | TransIT-VirusGEN® Transfection Reagent | 5 × 1.5 mL |
| MIR6706 | TransIT-VirusGEN® Transfection Reagent | 10 × 1.5 mL |
RevIT™ AAV Enhancer
Paired with any transfection reagent, the RevIT™ AAV Enhancer delivers higher titers for recombinant adeno-associated virus production.
Produce more patient doses at a lower cost and in less time.
High capacity, high yield, and high-quality virus production are essential for advancing the cell and gene therapy field. To increase the production of viral particles, you can scale up the bioreactor or increase the number of bioreactor runs. In other words, do more of the same process.
An alternative solution is to optimize your transfection process with the VirusGEN® transfection platform and RevIT™ AAV Enhancer. You’ll not only increase productivity, but achieve significant long-term time and cost savings.
This novel enhancer is easily integrated into existing workflows, and it produces 2-4x higher titers across a range of serotypes, cellular growth media formulations, and transfection platforms. RevIT™ AAV Enhancer can further drive down upstream manufacturing costs by permitting the use of lower levels of plasmid DNA while maintaining high-quality titers. Continual improvements in both upstream and downstream AAV manufacturing processes will allow the next generation of gene therapies to reach a broader audience.
| Product Number | Description | Pkg Size |
|---|---|---|
| MIR8000 | RevIT™ AAV Enhancer | 1.5 mL |
| MIR8006 | RevIT™ AAV Enhancer | 10 × 1.5 mL |
| MIR8007 | VirusGEN® AAV Kit + RevIT™ for 1L Culture | 1L Kit |
| MIR8008 | VirusGEN® AAV Kit + RevIT™ for 10L Culture | 10L Kit |
