***CUTANA™ CUT&Tag Kit, 48 Reactions (Primer Set 2)

The CUTANA™ CUT&Tag Kit offers a comprehensive solution for ultra-sensitive mapping of histone post-translational modifications (PTMs). Key advantages include:

• All-inclusive solution – The CUTANA™ CUT&Tag Kit includes all reagents needed to go from cells to sequencing libraries (including sequencing primers!).
• User-friendly workflow – Streamlined 8-strip tube workflow and exclusive direct-to-PCR protocol ensures increased throughput and minimal sample loss.
• Controls drive reliability – Control antibodies and nucleosome spike-ins enable assay optimization and continuous monitoring.
• Flexible pack sizes for every workflow – Support diverse research needs from first-time users to high-throughput studies by multiplexing up to 96 reactions.
• Extensive support library – The kit manual and Tech Support Center provide comprehensive guides for experimental design, quality checks, and FAQs.
• Stringent quality control – Each new lot undergoes rigorous quality control checks to ensure consistent results in your research.

In CUT&Tag, antibody-bound chromatin is selectively cleaved with fusion protein pAG-Tn5, which simultaneously adds sequencing adapters. Tagmented fragments bypass traditional library prep with CUTANA™ CUT&Tag Kit’s exclusive Direct-to-PCR strategy, enabling users to go from cells to PCR-amplified sequencing libraries in one tube with minimal sample loss. The recommended input for CUT&Tag is 100,000 native nuclei per reaction. Comparable data can be generated down to 10,000 nuclei, and the protocol is also validated for whole cells, cryopreserved samples, and lightly cross-linked nuclei or cells. CUT&Tag provides robust profiling for histone PTMs; for chromatin-associated proteins (e.g. transcription factors), CUTANA™ CUT&RUN is recommended.

The CUT&Tag Kit Version 4 (v4) now includes two new Wash Buffer Enhancers, CUTANA™ DNA Purification beads, and improvements to the CUT&Tag protocol. CUTANA™ DNA Purification Beads are optimized for high yield DNA clean up with precise size selection, while the CUTANA™ Wash Buffer Enhancers reduce clumping and improve bead handling. Protocol improvements amplify sample preservation with changes to nuclei resuspension and refine reaction handling to increase yields for difficult targets. The protocol is also designed for compatibility with multi-channel pipetting for increased throughput and reproducibility. Positive (H3K4me3 and H3K27me3) and negative (IgG) control antibodies are paired with the SNAP-CUTANA™ K-MetStat Panel of nucleosome spike-in controls to continuously monitor workflows and guide troubleshooting. CUTANA™ CUT&Tag Kits are bench-tested, scientist-approved, providing users with quality reagents for precision mapping.