***CUTANA™ Fragmented Controls for DNA Methylation Sequencing Set

The CUTANA™ Fragmented Controls for DNA Methylation Sequencing set includes pre-fragmented methylated pUC19 and unmethylated Lambda DNA controls optimized for assessing cytosine conversion efficiency in Enzymatic Methyl-seq (NEB® EM-seq™) when performed downstream of meCUT&RUN and Multiomic CUT&RUN workflows (CUT&RUN-EM). EM-seq is the preferred method for achieving base-pair resolution of 5-methylcytosine (5mC) from CUT&RUN DNA libraries. In traditional EM-seq, cytosine conversion controls are fragmented through sonication after mixing with genomic DNA. When CUT&RUN is used prior to EM-seq to excise chromatin regions of interest, pre-fragmented pUC19 and Lambda controls are required.

CUTANA Fragmented Controls for DNA Methylation Sequencing provides a reliable and easy-to-use solution for validating conversion rates and optimizing methylation sequencing protocols in cutting-edge epigenomics experiments.