**CUTANA™ Hia5 for Fiber-seq, 8 rxns

CUTANA™ Hia5 is the key enzyme for Fiber-seq, a multiomic long-read sequencing (LRS) assay that simultaneously profiles chromatin accessibility, DNA methylation, and genetic variation at single-molecule resolution. By layering chromatin accessibility on top of standard LRS workflows, Fiber-seq uniquely links epigenetic information (DNA methylation) and complex genetic architecture (e.g., structural variants and haplotype phasing) to chromatin states within the same molecules of DNA.

Hia5 is a DNA adenine methyltransferase (MTase) that uses S-adenosylmethionine (SAM) to catalyze methylation at the N6 position of adenine, producing N6-methyladenine (6mA). Fiber-seq leverages the nonspecific activity of Hia5 to methylate adenines within accessible chromatin regions in situ. This records chromatin accessibility information onto native DNA while preserving endogenous methylation (5mC). Hia5-modified DNA is fully compatible with LRS on both Pacific Biosciences® (PacBio® HiFi Sequencing) and Oxford Nanopore Technologies® (ONT® Nanopore Sequencing) platforms.

Features & Benefits:

• Multiomic insights in one assay – Fiber-seq simultaneously profiles chromatin accessibility, DNA methylation, and genetic variants, consolidating what typically requires three or more separate assays into one long-read sequencing assay.
• Highest activity 6mA-MTase  – Faster labeling via Hia5 enables higher resolution of accessible DNA, with demonstrated utility in protein footprinting applications to resolve transcription factor binding and nucleosome positioning.
• Access complex genomic regions – Profile chromatin accessibility in repetitive and structurally complex regions such as transposable elements, centromeres, telomeres, segmental duplications, and chromosomal rearrangements.
• Easy workflow integration – Fiber-seq can be performed in <2 hours directly upstream of routine long-read sequencing library preparation.
• Resolve chromatin state heterogeneity – PCR-free approach leverages long-read sequencing to directly profile individual DNA molecules, uncovering chromatin structure heterogeneity in cell populations often missed by short-read techniques.

Specifications:

• Type: Adenine Methyltransferase
• Molecular Weight: 33.8 kDa
• Host: E. coli
• Epitope Tag: 6xHis
• Storage: Stable for six months at -80ºC from date of receipt.
• Formulation: 50 mM Tris pH 8.0, 1 mM DTT, 250 mM NaCl, 10% glycerol