***CUTANA™ SsDddA Deaminase - 50 µL (100 µM)

SsDddA is a double-stranded DNA (dsDNA) cytidine deaminase from the bacterial species Simiaoa suniithat converts accessible cytosine to uracil [1]. These uracil residues can then be detected as C-to-T mutations following PCR amplification and sequencing. SsDddA has been used to label chromatin accessibility in two recently published methods, DAF-seq [1] and ACCESS-ATAC [2].

Double-stranded DNA deaminases are key enabling enzymes in emerging deaminase-based chromatin accessibility mapping methods. In general, cytosines that are bound by proteins or wrapped within nucleosomes are protected from deamination. After sequencing, stretches of DNA without C-to-T mutations are inferred to be protein-occupied. Because the resulting C-to-T marks are non-destructive to DNA and are preserved through PCR amplification, these methods are compatible with both long-read and short-read sequencing. When paired with long-read sequencing, deaminase-mediated labeling enables chromatin accessibility mapping and protein footprinting from lower sample inputs than other long-read accessibility assays that are not PCR-compatible. When paired with short-read sequencing, deamination provides more precise protein footprinting than standard ATAC-seq. Users interested in learning more about deaminase-based methods are encouraged to consult a recent review by Krebs et al. describing deaminase-based single-molecule genomics methods [3].

Key Advantages

Ready to Use

Commercially available SsDddA eliminates the need to express, purify, and validate your own dsDNA deaminase so you can focus on experimenting with the actual assay instead.

Quality Assured

SsDddA comes with a Certificate of Analysis verifying both purity and activity, reducing sources of experiment-to-experiment variability that can come with homebrewed reagents.

Exclusivity

EpiCypher is the only commercial provider of SsDddA, so your lab has reliable, ongoing access to reagents that cannot be sourced reliably elsewhere.