***CUTANA™ meCUT&RUN Kit for DNA Methylation Sequencing

Product Details

The CUTANA™ meCUT&RUN Kit for DNA Methylation Sequencing enables streamlined, high-resolution mapping of DNA methylation at >20-fold reduced sequencing depths compared to whole genome strategies like bisulfite sequencing.

In meCUT&RUN, a GST-tagged MeCP2 methyl binding domain binds methylated DNA, directing the selective cleavage and release of DNA methylation-enriched chromatin fragments into solution by an immunotethered nuclease (pAG-MNase). Enriched DNA is separated from bead-bound cells, purified, and prepared for sequencing by one of two methods.

The meCUT&RUN Kit is designed for 8-strip tubes and multi-channel pipetting, enabling a seamless workflow that maximizes throughput and reproducibility. The kit is compatible with a variety of inputs including cells or nuclei derived from native, cryopreserved, or cross-linked samples. While it is recommended to start with 500,000 cells, comparable data can be generated using as few as 10,000 cells. The low sequencing depth requirements, as well as compatibility with diverse sample inputs and low cell numbers, make this kit an ideal solution for DNA methylation sequencing.