***CUTANA™ Uncharged 6xHis-pAG-Tn5 for CUT&Tag, 75 µL

Products in EpiCypher’s IDEA Toolbox (Innovation and Discovery of Epigenetic Applications) offer access to reagents without known or fully defined uses, enabling researchers to explore cutting-edge applications. Due to their novelty and unexplored potential, EpiCypher will engage in limited technical support.

CUTANA™ Uncharged 6xHis-pAG-Tn5 is a fusion of proteins A and G to E. coli transposase (Tn5), the key enzyme for CUT&Tag. The fusion protein contains a polyhistidine tag (6xHis) to enable applications that require affinity purification steps (for an untagged version of uncharged pAG-Tn5, see Product Number EP151025). Uncharged Tn5 must be loaded with user-designed mosaic adapter DNA prior to use in CUT&Tag.

His-pAG-Tn5 enables purification of differentially barcoded antibody-Tn5 complexes such as those described in MulTI-Tag (Multiple Target Identification by Tagmentation) and multi-CUT&Tag workflows to simultaneously interrogate multiple chromatin proteins in a single CUT&Tag reaction. These approaches have been applied to map histone post-translational modifications and RNA Polymerase II down to single cell resolution to define cell-specific epigenetic landscapes.