***CUTANA™ Uncharged pAG-Tn5 for CUT&Tag, 20 µL

Datasheet

CUTANA™ Uncharged pAG-Tn5 is a fusion of proteins A and G to E. coli transposase (Tn5) and is the key enzyme for CUT&Tag. This product is highly purified to remove contaminating E. coli DNA. For superior normalization, as well as antibody validation and reaction monitoring, SNAP-CUTANA™ nucleosome spike-ins (e.g. EP191002) are recommended. This Tn5 is uncharged and must be loaded with user-designed mosaic adapter DNA prior to use in CUT&Tag. The uncharged protein exists as a monomer but will dimerize when charged with DNA. For a charged enzyme that is ready to use, see product EP151017.