EC HPLC Column, NUCLEODUR 100-5 NH2-RP, 5 µm, 125x4 mm

This HPLC column is packed with polar aminopropyl modification, based on high purity NUCLEODUR silica gel. This HPLC phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions.

• Method: HPLC, analytical
• Brand: NUCLEODUR
• Base material: NUCLEODUR - high purity silica
• Phase: NUCLEODUR NH2-RP 100-5
• USP listing: L8
• Surface chemistry: Aminopropyl (NH2) modification
• Mode Multi-mode: (NP and RP), Reversed phase (RP)
• Type: EC HPLC column (analytical)
• Hardware: Stainless steel
• Column length: 125 mm
• Column inner diameter: 4 mm
• Particle type: Fully porous particles (FPP)
• Particle shape: Spherical
• Particle size: 5 µm
• Eluent in HPLC column: Acetonitrile – water
• Recommended application(s): HPLC, Hydrocarbons under NP conditions, Polar compounds under RP conditions (sugars, DNA bases)
• Endcapped: No
• Carbon content: 2.5 %
• pH stability: 2.0–8.0
• Pore size: 110 Å
• Specific surface according to BET: 340 m²/g
• Temperature stability (max): 60 °C
• Pressure stability (max): 600 bar / 8700 psi
• Column volume: 1.56 mL
• Storage temperature: 15–25 °C / 59–77 °F
• Scope of delivery: 1 x analytical HPLC column, instruction leaflet, certificate of analysis