EVlution™ Digital TFF System — Scalable Exosome and EV Purification by Tangential Flow Filtration
EVlution™ is a compact benchtop tangential flow filtration (TFF) instrument that provides efficient size-dependent concentration and purification of biomolecules, including extracellular vesicles (EVs). EVlution utilizes the innovative Switch Flow™ system to generate sub-10 ml samples from large starting volumes.
Developed and supported by scientists with decades of experience in separation technology, EVlution™ TFF provides an effective method of size-based separation of biomolecules, whilst minimizing membrane fouling. The innovative characteristics of EVlution™ design make it an excellent choice for workflows at the lab scale, supporting a wide range of hollow fibre filter configurations. EVlution™ simplifies complex filtration processes while delivering reliable performance.
For concentrating extracellular vesicles (EVs), 300 or 500 kDa MWCO cartridges are recommended.
EVlutionTMDigital System includes everything in the standard EVlution system plus a digital pressure gauge
- The EVlution stand
- The EVlution pump
- The EVlution digital pressure gauge
- A 94cm2 cartridge. Choose from 5 kDa to 1000 kDa
- A set of syringes
- A set of tubing and valves
- All accessories (mounting clips and pressure gauge housing)
Note: Duran bottles are NOT included.
Product Details:
- Storage Temperature: Room temperature
- Processable Volume: Up to 1 litre with 94 cm2 cartridge included (3 litres with 254 cm2 cartridge, available separately)
- Supplied Cartridge: area 94 cm2
- Cartridge Pore Size: Choose any pore size from 5 kDa to 1000 kDa
- Pumping Capacity: 0.1 - 86 ml/min
- Pressure Gauge Units: Select between psi, bar, MPa, and kg/cm, 1% accuracy,
- Footprint: 21 cm x 26.5 cm
- System Weight: 2.7 kg
Product Attributes:
Hollow fibre technology. EVlution uses hollow fibre filter cartridges to provide the low-shear, laminar flow environment that extracellular vesicles need. This preserves vesicle integrity and biological function, giving you confidence that what you isolate faithfully represents what was in your sample.
Innovative Switch Flow™ for the smallest output volumes. The ability to switch from circulated to reversible retentate flow enables a final retentate volume below 10 ml
Digital pressure gauge. Accurately measure pressure in: bar (to two decimal points), mPA, psi, kg/cm2
Fully displaceable sample contact surfaces. By incorporating a disposable syringe-based pressure gauge, we have made all surfaces that the sample flows through disposable, allowing easy maintenance.
Genuinely affordable. EVlution is accessible to research groups, core facilities and teaching laboratories.
Compact benchtop footprint. With dimensions of just 21 × 26.5 cm and a weight of 2.7 kg, EVlution takes up less bench space than a standard laboratory balance. It requires no dedicated infrastructure - just plug in and go.
Flexible filter configurations. The adaptable design supports a range of hollow fibre cartridges from 5 kDa or 1000 kDa MWCO options, along with different cartridge sizes (94 cm² and 260 cm² membrane area). This allows you to match the system to your specific application, up to 3 litres.
Simple, intuitive operation. EVlution has been designed for ease of use. Researchers can set up and run a TFF process without extensive training or bioprocessing expertise. The streamlined workflow lowers the barrier to adoption and reduces the risk of operator error.
Proven protein removal performance for EV processing. EVlution significantly reduces the concentration of free proteins in the retentate during EV concentration, as shown by BCA protein assay, while NTA particle tracking confirms efficient recovery of EV particles. This dual evidence of concentration and purification in a single step saves time and reduces sample handling.
Part of a complete EV workflow. Cell Guidance Systems offers a comprehensive EV product portfolio, including Exo-spin™ SEC columns, ExoLISA™ assays, validated EV antibodies, purified reference exosomes, and NTA profiling services. EVlution integrates seamlessly as the front-end concentration and initial purification step in a multi-stage workflow — for example, TFF followed by Exo-spin SEC for high-purity exosome isolation.
Backed by EV expertise. Cell Guidance Systems is a specialist in extracellular vesicle research tools. When you purchase EVlution, you gain access to a team with deep knowledge of EV biology and purification who can advise on protocol optimisation and troubleshooting.
