The LNP formulation loaded with eGFP mRNA (m1Ψ) is prepared using generic LNP formulation with main ionizable lipid of ALC0315. The payload EGFP mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudouridine (m1Ψ). It expresses an enhanced green fluorescent protein, with the open reading frame sequence originally from the jellyfish, Aequorea Victoria. EGFP mRNA is a commonly used fluorescence reporter sequence in mRNA therapeutics and delivery system development projects. The expressed protein has an excitation wavelength of 488nm, emission at 507nm. This formulation is a good control for testing if ALC0315 LNP formulation is a good potential formulation for your mRNA product under research in your in vitro and in vivo experiment model.
Specifications:
Form: Liquid
Concentration: 0.15mg/mL
Full mRNA length: 980nt
Full mRNA Molecular Weight: 319792
Storage buffer: Tris-HCl/Sucrose
Storage condition: Store at -80°C for long term. Avoid freeze thaw, only thaw once before use.