SKU: VECTGEK1000Available Quantity : 0
The Glysite™ Explorer in situ PLA Glycan Detection Kit is a fully integrated tool for the spatial detection of glycans proximal to a protein of interest in FFPE tissues, FFPE cell pellets and fixed cells. This system integrates a curated panel of Vector’s Glysite™ Explorer Lectins with Navinci’s proprietary in situ Proximity Ligation Assay (isPLA). Researchers can flexibly select specific Glysite™ Explorer Lectins covering the major categories of glycan types to pair with a primary antibody targeting their protein of interest. This provides a simple approach to understand glycan-protein proximity with a spatial context.
The Glysite™ Explorer in situ PLA Glycan Detection Kit (GEK-1000) provides all core reagents needed for the workflow. To complete your assay, please select at least one Glysite™ Explorer Lectin (up to 10) and supply your primary antibody of choice.
Glysite™ Explorer in situ PLA Glycan Detection Kit contains the following reagents:
Box 1 Reagents(4°C storage, do not freeze):
- BLOXALL® Blocking Solution
- Blocking Buffer 1
- Blocking Buffer 2A
- Blocking Buffer 2B (25x)
- Protein Diluent
- Lectin Probe (50x)
- Mouse Probe (50x)
- Rabbit Probe (50x)
- ImmPRESS® HRP Reagent (400x)
- ImmPRESS® HRP Diluent
- ImmPACT® DAB Reagent (33x)
- ImmPACT® DAB Diluent
- Hematoxylin QS
Box 2 Reagents(-20°C storage):
- Buffer 1 (5x)
- Enzyme 1 (40x)
- Buffer 2 (5x)
- Enzyme 2 (40x)
