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**Glysite™ Explorer in situ PLA Glycan Detection Kit

**Glysite™ Explorer in situ PLA Glycan Detection Kit

SKU: VECTGEK1000
Available Quantity : 0

Product Details

The Glysite™ Explorer in situ PLA Glycan Detection Kit is a fully integrated tool for the spatial detection of glycans proximal to a protein of interest in FFPE tissues, FFPE cell pellets and fixed cells. This system integrates a curated panel of Vector’s Glysite™ Explorer Lectins with Navinci’s proprietary in situ Proximity Ligation Assay (isPLA). Researchers can flexibly select specific Glysite™ Explorer Lectins covering the major categories of glycan types to pair with a primary antibody targeting their protein of interest. This provides a simple approach to understand glycan-protein proximity with a spatial context.  

The Glysite™ Explorer in situ PLA Glycan Detection Kit (GEK-1000) provides all core reagents needed for the workflow. To complete your assay, please select at least one Glysite™ Explorer Lectin (up to 10) and supply your primary antibody of choice.

Glysite™ Explorer in situ PLA Glycan Detection Kit contains the following reagents:

Box 1 Reagents (4°C storage, do not freeze):

  • BLOXALL® Blocking Solution
  • Blocking Buffer 1
  • Blocking Buffer 2A
  • Blocking Buffer 2B (25x)
  • Protein Diluent
  • Lectin Probe (50x)
  • Mouse Probe (50x)
  • Rabbit Probe (50x)
  • ImmPRESS® HRP Reagent (400x)
  • ImmPRESS® HRP Diluent
  • ImmPACT® DAB Reagent (33x)
  • ImmPACT® DAB Diluent
  • Hematoxylin QS

Box 2 Reagents (-20°C storage):

  • Buffer 1 (5x)
  • Enzyme 1 (40x)
  • Buffer 2 (5x)
  • Enzyme 2 (40x)
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