**Glysite™ Explorer in situ PLA Glycan Detection Kit

The Glysite™ Explorer in situ PLA Glycan Detection Kit is a fully integrated tool for the spatial detection of glycans proximal to a protein of interest in FFPE tissues, FFPE cell pellets and fixed cells. This system integrates a curated panel of Vector’s Glysite™ Explorer Lectins with Navinci’s proprietary in situ Proximity Ligation Assay (isPLA). Researchers can flexibly select specific Glysite™ Explorer Lectins covering the major categories of glycan types to pair with a primary antibody targeting their protein of interest. This provides a simple approach to understand glycan-protein proximity with a spatial context.  

The Glysite™ Explorer in situ PLA Glycan Detection Kit (GEK-1000) provides all core reagents needed for the workflow. To complete your assay, please select at least one Glysite™ Explorer Lectin (up to 10) and supply your primary antibody of choice.

Glysite™ Explorer in situ PLA Glycan Detection Kit contains the following reagents:

Box 1 Reagents(4°C storage, do not freeze):

• BLOXALL® Blocking Solution
• Blocking Buffer 1
• Blocking Buffer 2A
• Blocking Buffer 2B (25x)
• Protein Diluent
• Lectin Probe (50x)
• Mouse Probe (50x)
• Rabbit Probe (50x)
• ImmPRESS® HRP Reagent (400x)
• ImmPRESS® HRP Diluent
• ImmPACT® DAB Reagent (33x)
• ImmPACT® DAB Diluent
• Hematoxylin QS

Box 2 Reagents(-20°C storage):

• Buffer 1 (5x)
• Enzyme 1 (40x)
• Buffer 2 (5x)
• Enzyme 2 (40x)