HighPrep HMW DNA Kit (96 preps)

HighPrep HMW DNA Kit Protocol

The HighPrep High Molecular Weight DNA Kit is specifically designed for isolation of high molecular weight DNA (HMW DNA) in the size range of 50–300+ kb. The kit extracts HMW DNA from biological samples such as whole blood, bone marrow, saliva, buccal cells, cultured cells, tissues, and bacteria. The HighPrep High Molecular Weight DNA Kit utilizes magnetic bead-based technology in combination with chaotropic agents to gently isolate HMW DNA and remove inhibitors. The HMW DNA purifi ed with this kit is particularly suitable for analysis on long read sequencing genomic platforms including PacBio RSII/Sequel/Sequel II and Oxford Nanopore. DNA purifi ed using the HighPrep High Molecular Weight DNA Kit is of good quality and purity. The kit includes RNase A for the removal of RNA. The exact size of extracted DNA varies depending on sample matrix, the quality of the starting material, and processing conditions.

• Isolation of high-quality, high molecular weight DNA 50-300+ kb
• HMW DNA is suitable for all third-generation sequencing platforms including Nanopore and PacBio SMRT sequencing
• Achieve high yields of DNA from a wide range of sample matrices
• Efficient scale-up and flexibility
• Amenable to automation and has easy to use protocols (Kingfisher™ scripts are ready)
• Comes with RNase to minimize the co-purification of RNA