*HOOK™ Agarose Coupling Kit (Amine Reactive), For 5 Columns

Protocol

• The amine reactive HOOK Agarose Coupling Kit is designed for the simple and efficient coupling of proteins and peptides to a solid support.
• The kit allows the generation of both a stable and reusable column for the affinity purification of antibodies, proteins and other protein interacting biomolecules.
• This kit utilizes 6% agarose that has been activated to generate reactive aldehyde groups.
• The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base complexes. These, in turn, are selectively reduced by reductive amination to form stable amine linkages between the agarose and the ligand.