HOOK™ Peptide Coupling Kit (Amine reactive) with OneQuant™ KLH, 5 Reactions

Protocol

• The HOOK Amine Reactive Peptide Coupling Kit is designed for the coupling of peptides to carrier proteins, utilizing the primary amines and carboxyl groups of the peptides and carrier proteins.
• This kit utilizes the chemical heterobifunctional crosslinker EDC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) to couple peptides to carrier proteins.
• EDC first reacts with the carboxyl groups, producing an amine reactive intermediate, O-acylisourea that rapidly reacts with the amine groups of the peptide/protein.
• Simple to use
• Suitable for all carrier proteins
• Utilizes Tube-O-Reactor for minimum hands on time