HOOK™ peptide Coupling Kit (Sulfhydryl reactive) with OneQuant™ KLH, 5 Reactions

Protocol

• The HOOK Sulfhydryl Reactive Peptide Coupling Kit is designed for the coupling of peptides to carrier proteins, utilizing a sulfhydryl group in the peptide.
• This kit exploits the chemical crosslinker sulfoSMCC (Sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) to couple peptides through their sulfhydryl groups, found on cysteine side chains, to the primary amines on the carrier proteins.
• The Nhydroxysuccinimide (NHS) ester in sulfoSMCC reacts with primary amines to form covalent amide bonds and the maleimide group reacts with sulfhydryl groups to form stable thioether bonds.