HOT FIREPol® Probe qPCR Mix Plus (ROX), 20 mL (5000 rxn)

Product Details

Datasheet

• HOT FIREPol® Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe.
• The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® Probe qPCR Mix Plus (ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity.
• HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
• Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
• Cost-effective real-time qPCR master mix for probe-based qPCR assays.
• This master mix has been developed for TaqMan® probes but is suitable for other hydrolysis probe types as well.
• Suitable for singleplex and duplex assays
• High specificity and sensitivity
• Reaction set-up and shipment without dry ice