Jacalin, Fluorescein labeled

Product Details

Jacalin, Fluorescein, 5 mg, is a conjugated form of Jacalin, a lectin that comprises four subunits of approximately 16 kDa each. Jacalin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine, also known as the T-antigen. This is the oligosaccharide to which peanut agglutinin (PNA) binds but, unlike PNA, jacalin will bind a mono- or disialylated form of this structure. Jacalin has been used to purify human IgA and its specificity also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains. Fluorescein-labeled jacalin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin and is supplied essentially free of unconjugated fluorochromes. Fluorescein has an excitation maximum of 495 nm and an emission maximum of 515 nm.