**Mononucleosomes, Recombinant, Symmetrically Methylated 199x601 DNA,Biotinylated

Product Details

Mononucleosomes assembled from recombinant histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P06897; H2B-P02281; H3-Q92133; H4-P62799) wrapped by 199 base pairs of DNA containing the 601 positioning sequence DNA. The the 199bp DNA sequence contains a centrally positioned 601 sequence, identified by Lowary and Widom, which is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly. The 601 sequence is flanked by a symmetrically methylated 26 bp sequence as shown in application notes and contains a 5' biotin-TEG group.