This kit is based on capture ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti Mouse IgM(U-chain). HRP-antigen is used as detection antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-antigen. Then, it binds with Anti-KLH IgM bound to anti Mouse IgM(U-chain). Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Anti-KLH IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450.
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