The PDQ™ Protease Assay is a unique colorimetric assay used to detect protease activity in aqueous samples. The proprietary substrate responds to a wide range of proteases including serine, metallo, aspartate and cysteine proteases such as collagenase, proteinase K, papain, pepsin, bromelin, ficin, trypsin and chymotrypsin. PDQ™ can be used with just a few simple steps to measure protease activity and requires no centrifugation. The substrate is a cross-linked matrix containing protein substrate and a dye-protein conjugate. Protease activity is detected spectrophotometrically with increasing optical density proportional to increasing enzyme activity and can detect nanogram quantities.
Each kit is supplied with a trypsin solution for generating standard curves (BAEE equivalent units). PDQ™ is supplied in 48 ready-to-use plastic vials.