PN3712 Online DLS

Features

High Sensitivity Online Dynamic Light Scattering Detector Add-on Module, especially for connection to MALS detector cell as subsequent detector for Size Exclusion and Field-Flow Fractionation (FFF) separation. Possibility to perform online DLS and MALS measurement to obtain Particle Size (Rh//Rg ) and Molecular Weight (MW) in the same scattering volume in one flow cell.

Online DLS Detector Module

The new PN3712 advanced online DLS module can be directly linked to any PN3600 series MALS detector. It operates with state-of-the–art industry leading photodetector hardware and a powerful hardware correlator. The correlator has been especially developed to meet the requirements of complex samples such as biopharma, polymer and particle systems with significant heterogeneity and high diluted lower concentrated samples. The PN3712 Online DLS module allows direct operation of Online Mode DLS inside any PN3600 MALS flow cell and can be connected to any angle in the range of 44° - 140° and connected to upfront SEC and FFF separation. The module allows to continuously determine correlation functions and Rh values in the same flow cell volume where the MALS static light scattering data for calculation of Rg and MWis acquired without dilution effects.

Online DLS Software Module

The advanced DLS control software for operation in connection with Nova software allows automated operation and ease of use for any operators. Software allows specific selection of DLS parameter including calculation model for advanced DLS research application. By using the standards settings software also can be used in routine mode for generating DLS and Rh data for standard measurements. Software also allows post-adjustment of processing parameters in order to obtain most meaningful DLS data. Data will be stored additional as ASCII / CSV files and thus be exported to other external software packages, such as Matlab, Origin, etc. for further evaluation and processing. Several parameters can be exported additional to Rh, such as temperature, DLS settings and intercept.

Online Mode Specifications

• Particle size range (Rh): 0.5 - 500 nm*
• Typical SEC/FFF flow rate range: 0.05 to 1 mL/min
• Pressure range: maximum 5 bar differential pressure at cuvette inlet/outlet

* Depending on sample material, concentration and SEC/FFF flow rate