S100A4, Human, ELISA kit, 2 x 96 det.

SKU: HYBHK37702

Available Quantity : 0

The Human S100A4 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.
The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay.
Samples and standards are incubated in microtiter wells coated with antibodies recognizing Human S100A4.
Biotinylated tracer antibody will bind to the captured Human S100A4.
Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody.
Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB).
The enzyme reaction is stopped by the addition of oxalic acid.
The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Human S100A4 standards (log).
The Human S100A4 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve

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