Lyo-ready RT-qPCR solution optimized for effective lyophilization to beads or cakes
Offers flexibility on the choice of excipients and input template (DNA or RNA)
Competitive critical temperatures and low residual moisture content
Reconstitution of the lyophilizate in seconds
Low-copy number target detection
Preserved performance after lyophilization
SOLIScript® Lyo-ready 1-step RT-qPCR Kit is an optimized solution for fast and sensitive amplification and quantitation of RNA and DNA targets with probe-based assays. With a high collapse temperature (Tc) of -30 ˚C, the kit allows for the adoption of a rapid and energy-efficient lyophilization protocol. With a glass transition temperature (Tg) of 85.9˚C, the lyophilizates have a high resistance against long term exposure to higher temperatures. The composition is optimized for lyophilization to cakes or beads. The kit comes in 4 tubes: 40x SOLIScript® Lyo-compatible One-Step Mix, HOT SolisFAST® Glycerol-Free DNA Polymerase (10 U/μl), 4x Lyo Reaction Buffer and 4x RT-qPCR Lyo Excipient Mix.
40x SOLIScript® Lyo-compatible One-Step Mix is a 40x concentrated RT mix that includes an in silico designed SOLIScript® reverse transcriptase and RiboGrip® RNase Inhibitor. SOLIScript® is a thermostable reverse transcriptase active at temperatures up to 60°C, beneficial when using templates with high levels of secondary structure. RiboGrip® inactivates RNase A to protect RNA sample from degradation and increase cDNA yield in reactions with low RNA amounts.
HOT SolisFAST® Glycerol-Free DNA Polymerase (10 U/μl) is an in silico designed inhibitor tolerant analogue of Taq DNA polymerase with chemical hot-start and ~2-4 times faster extension rates compared to the wild-type Taq.
4x Lyo Reaction Buffer contains additives for efficient amplification. The MgCl2 concentration is 20 mM (1x mix – 5 mM).
4x RT-qPCR Lyo Excipient Mix is a 4x concentrated blend of lyophilization additives that protect reagents during freeze-drying and stabilize the lyophilizates. The mix enables lyophilization into both cakes and beads.
Reagents Provided:
40x SOLIScript® Lyo-compatible One-Step Mix, 2.5 ml
HOT SolisFAST® Glycerol-Free DNA Polymerase (10 U/μl), 2 ml
4x Lyo Reaction Buffer, 25 ml
4x RT-qPCR Lyo Excipient Mix, 25 ml
Storage:
Routine storage: -20 ºC.
Temporary storage for up to 2 weeks at room temperature has no detrimental effects on the quality of SOLIScript® Lyo-ready 1-step RT-qPCR Kit.
