Soybean agglutinin (SBA), Agarose bound

Product Details

Soybean Agglutinin (SBA), Agarose bound, 2 ml, is an agarose-conjugated form of SBA. SBA is a family of closely related isolectins, each comprising four subunits of approximately equal size. This glycoprotein has a molecular weight of about 120 kDa. It preferentially binds to oligosaccharide structures with terminal alpha- or β-linked N-acetylgalactosamine. Covalent attachment of SBA to the agarose beads (using a hydrophilic spacer arm) preserves lectin activity and minimizes conformational changes that might result in non-specific or hydrophobic interactions. This also ensures that conjugated proteins are not leached off the beads by Tris or other routinely used buffers. This product is supplied as a 1:1 suspension in buffer and comprises a matrix of heat-stable, crosslinked 4% agarose beads that is stable in solutions at pH 3–11 as well as many organic solvents. Because no residual charge is present after conjugation, non-specific binding to the matrix is minimized.