*TransIT-PRO® Transfection Kit – 1.0 mL (with boost)

Ideal for Biotherapeutic Protein Production in Suspension CHO and 293 Cells

Reach critical stages faster. Decrease time to produce usable protein by maximizing target protein yields through transient transfection. The TransIT-PRO® Transfection Reagent & Kit use animal origin free components designed for high and reproducible protein yield in suspension CHO and 293 derived cells. Since it is compatible with varied media formulations, the same media can be used for both transient and stable expression. Use with CHOgro® Expression Media for highest yield in suspension CHO cells. TransIT-PRO® outperforms linear PEI in protein yield, while providing a cost-effective alternative to FreeStyle™ MAX.

• High Protein Yield – in Suspension CHO and 293 cells, including CHO-S and Expi293F™ cells
• Compatible with Multiple CHO Media Formulations – Use with CHOgro® Expression Medium to Achieve Highest Yields
• Simple Workflow – Reproducible Protein Expression with Minimal Optimization

Storage Conditions: All Configurations: Store at -20°C

Materials Supplied:

• TransIT-PRO® Reagent 1 × 1 ml
• PRO Boost Reagent 1 × 1.5 ml

Materials Required, but Not Supplied

• Protein production cell line
• Culture vessels
• Appropriate cell culture medium
• Purified, endotoxin-free DNA
• Serum-free medium
• Sterile tube for transfection complex preparation
• Orbital shaker
• Reporter assay as required