VectaCell Trolox

VectaCell Trolox Antifade Reagent for Live Cell Imaging, 2 ml, is a stock solution of the antioxidant Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) in filtered ethanol. Trolox is a water-soluble and cell-permeable analog of vitamin E that efficiently prevents formation of reactive oxygen species such as singlet oxygen (1O2), superoxide anion (O2-), or hydrogen peroxide (H2O2). These can lead to photo-bleaching as well as oxidative damage in cells. While reducing the light intensity usually decreases fading, it also reduces the assay sensitivity. This product contains both Trolox and its oxidized form Trolox-quinone. Trolox has been shown to have a cytoprotective effect and low cytotoxicity for different cell lines and this redox system has been shown to reduce photo-bleaching and blinking during live cell imaging.