Wheat germ agglutinin (WGA), Agarose bound

Product Details

Wheat Germ Agglutinin (WGA), Agarose bound, 10 ml. This product is prepared using 4% agarose beads. The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to its dimers and trimers. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Features: Bead diameter from 45–165 microns, Matrix is stable in solutions at pH 3–11 and many organic solvents, Immobilized lectins are prepared using affinity-purified lectins. Covalent attachment preserves lectin activity, minimizing conformational changes that might result in non-specific or hydrophobic interactions. Conjugated proteins are not leached off the beads by routinely used buffers. No residual charges are present after conjugation, minimizing non-specific binding to the matrix. Product supplied as a 1:1 suspension in buffer. Inhibiting/Eluting Sugar: Chitin Hydrolysate or 500 mM N-acetylglucosamine.